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Product Name
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Osteopontin antibody
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See all Osteopontin antibodies (15)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Osteopontin
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Immunogen
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Recombinant full length protein (Mouse) expressed in NSO cells.
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Reacts with
(species key)
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Ms
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Tested applications
(see key)
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ELISA, ICC/IF, Neut, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 0.5-1 µg/ml (indirect method).
ICC/IF: 1/50. (Based on Abreview.)
Neut: Use at a concentration of 1-3 µg/ml.
WB: Use at a concentration of 0.1-0.2 µg/ml. Detects a doublet band of approximately 65 kDa and a 30 kDa band (due to glycosylation).
The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Tested with 293 cells.
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Cellular localization
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Secreted
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Research areas
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Cancer >> Tumor biomarkers >> Other
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
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Relevance
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Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine.
Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 20750 (Mouse)
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Osteopontin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Osteopontin antibody (ab11503)
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This product has been used in:
Yang X et al. Bone morphogenic protein 2 induces Runx2 and osteopontin expression in human aortic valve interstitial cells: role of Smad1 and extracellular signal-regulated kinase 1/2. J Thorac Cardiovasc Surg 138:1008-15 (2009). PubMed: 19664780
If you publish research using ab11503 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Osteopontin
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Osteopontin antibody - more information
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Customer reviews (feedback) regarding Osteopontin antibody |
Customer FAQs regarding Osteopontin antibody |
Protocols for Osteopontin antibody |
Price and availability of products related to Osteopontin antibody |
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Osteopontin antibody - related products:
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Osteopontin antibody - other tools:
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Contact Abcam with a Technical Enquiry about Osteopontin antibody |
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