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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Overview
Product name
Anti-Osteopontin antibody
See all Osteopontin products (17) ...
Description
Goat polyclonal to Osteopontin
Tested applications
ICC/IF, WB, Neutralising, Indirect ELISA, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Recombinant full length protein (Mouse) expressed in NSO cells.
Positive control
Mouse osteopontin expressed in NSO cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/50
WB: Use a concentration of 0.1 - 0.2 µg/ml.Detects a band of approximately 65 kDa.
Neut: Use a concentration of 1 - 3 µg/ml.(The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells.)
I-ELISA: Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 17224478)
Target
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Tissue specificity
Bone. Found in plasma.
Sequence similarities
Belongs to the osteopontin family.
Post-translational
modifications
Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium.
Cellular localization
Secreted.
Target information above from: UniProt accessionP10451
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BNSP antibody
- Bone sialoprotein 1 antibody
- BSP I antibody
see all
Database links
- Entrez Gene: 20750 Mouse
- SwissProt: P10923 Mouse
- Unigene: 288474 Mouse
References for Anti-Osteopontin antibody (ab11503)
This product has been referenced in:
- Redding KMet al. Transgenic mice expressing an intracellular fluorescent fusion of angiotensin II demonstrate renal thrombotic microangiopathy and elevated blood pressure. Am J Physiol Heart Circ Physiol 298:H1807-18 (2010). WB; Mouse.Read more (PubMed: 20363893) »
- Yang Xet al. Bone morphogenic protein 2 induces Runx2 and osteopontin expression in human aortic valve interstitial cells: role of Smad1 and extracellular signal-regulated kinase 1/2. J Thorac Cardiovasc Surg 138:1008-15 (2009).Read more (PubMed: 19664780) »
See all 3 publications for this product
Publishing research using ab11503? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"