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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Overview
Product name
Anti-Osteopontin antibody
See all Osteopontin products (17) ...
Description
Rabbit polyclonal to Osteopontin
Specificity
There were no cross reactivities obtained with human Osteonectin, and human Bone Sialoprotein.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: PSKSNESHDHMDDMDD, corresponding to amino acids 59-74 of Human Osteopontin
PSKSNESHDH MDDMDD
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole Serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution.
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P
IHC-P: 1/1500IHC-P: 1/1500
Target
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Tissue specificity
Bone. Found in plasma.
Sequence similarities
Belongs to the osteopontin family.
Post-translational
modifications
Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium.
Cellular localization
Secreted.
Target information above from: UniProt accessionP10451
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BNSP antibody
- Bone sialoprotein 1 antibody
- BSP I antibody
see all
Database links
- Entrez Gene: 6696 Human
- Entrez Gene: 20750 Mouse
- Entrez Gene: 25353 Rat
- Omim: 166490 Human
- SwissProt: P10451 Human
- SwissProt: Q4W597 Human
- SwissProt: Q567T5 Human
- SwissProt: P10923 Mouse
see all
References for Anti-Osteopontin antibody (ab14175)
This product has been referenced in:
- Lavoie JFet al. Skin-derived precursors differentiate into skeletogenic cell types and contribute to bone repair. Stem Cells Dev 18:893-906 (2009).Read more (PubMed: 18834279) »
See 1 publication for this product
Publishing research using ab14175? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"