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Western blot - Osteopontin antibody (ab33046)
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Immunohistochemistry (Paraffin-embedded sections) - Osteopontin antibody (ab33046)
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Product Name
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Osteopontin antibody
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See all Osteopontin antibodies (15)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Osteopontin
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Immunogen
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A region within synthetic peptide: LSKVSREFHS HEFHSHEDML VVDPKSKEED KHLKFRISHE LDSASSEVN, corresponding to C terminal amino acids 252-300 of Human Osteopontin
BLAST 'LSKVSREFHS HEFHSHEDML VVDPKSKEED KHLKFRISHE ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1562500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Predicted molecular weight: 35 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: Jurkat cell lysate.
IHC-P: Human kidney.
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Cellular localization
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Secreted
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Research areas
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Cancer >> Tumor biomarkers >> Other
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
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Relevance
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Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine.
Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Osteopontin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Osteopontin antibody images, references, products related to ab33046 and other tools.
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Osteopontin antibody images:
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 Western blot - Osteopontin antibody (ab33046)
Osteopontin antibody (ab33046) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti Rabbit IgG at 1/50000 dilution
Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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Immunohistochemistry (Paraffin-embedded sections) - Osteopontin antibody (ab33046)
Ab33046, at a concentration of 4 µg/ml, staining Osteopontin in paraffin embedded human kidney tissue by Immunohistochemistry. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.
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References for Osteopontin antibody (ab33046)
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This product has been used in:
Jakobsen LP et al. Expression analyses of human cleft palate tissue suggest a role for osteopontin and immune related factors in palatal development. Exp Mol Med 41:77-85 (2009). PubMed: 19287188
If you publish research using ab33046 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Osteopontin
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Osteopontin antibody - more information
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Customer reviews (feedback) regarding Osteopontin antibody |
Customer FAQs regarding Osteopontin antibody |
Protocols for Osteopontin antibody |
Price and availability of products related to Osteopontin antibody |
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Osteopontin antibody - related products:
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Osteopontin antibody - other tools:
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Contact Abcam with a Technical Enquiry about Osteopontin antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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