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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Overview
Product name
Anti-Osteopontin antibody
See all Osteopontin products (17) ...
Description
Rabbit polyclonal to Osteopontin
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow
Immunogen
A region within synthetic peptide: LSKVSREFHS HEFHSHEDML VVDPKSKEED KHLKFRISHE LDSASSEVN, corresponding to C terminal amino acids 252-300 of Human Osteopontin
LSKVSREFHS HEFHSHEDML VVDPKSKEED KHLKFRISHE LDSASSEVN
Positive control
WB: Jurkat cell lysate. IHC-P: Human kidney.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1562500ELISA: 1/1562500
WB: Use a concentration of 1.25 µg/mlPredicted molecular weight: 35 kDa.
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Tissue specificity
Bone. Found in plasma.
Sequence similarities
Belongs to the osteopontin family.
Post-translational
modifications
Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium.
Cellular localization
Secreted.
Target information above from: UniProt accessionP10451
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BNSP antibody
- Bone sialoprotein 1 antibody
- BSP I antibody
see all
Database links
- Entrez Gene: 281499 Cow
- Entrez Gene: 6696 Human
- Entrez Gene: 20750 Mouse
- Omim: 166490 Human
- SwissProt: P31096 Cow
- SwissProt: Q56NM9 Cow
- SwissProt: Q58DM6 Cow
- SwissProt: P10451 Human
see all
Anti-Osteopontin antibody images:
Western blot - Osteopontin antibody (ab33046)
Anti-Osteopontin antibody (ab33046) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti Rabbit IgG at 1/50000 dilution
Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Immunohistochemistry (Paraffin-embedded sections) - Osteopontin antibody (ab33046)
Ab33046, at a concentration of 4 µg/ml, staining Osteopontin in paraffin embedded human kidney tissue by Immunohistochemistry. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.
References for Anti-Osteopontin antibody (ab33046)
This product has been referenced in:
- Jakobsen LPet al. Expression analyses of human cleft palate tissue suggest a role for osteopontin and immune related factors in palatal development. Exp Mol Med 41:77-85 (2009).Read more (PubMed: 19287188) »
See 1 publication for this product
Publishing research using ab33046? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"