Osteoprotegerin Mouse ELISA Kit (ab100733)
- Product nameOsteoprotegerin Mouse ELISA KitSee all Osteoprotegerin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 1 pg/ml
- Range1.65 pg/ml - 1200 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 88.7 81% - 102% Serum 91.7 82% - 104% Plasma 81.9 65% - 89%
- Species reactivityReacts with: Mouse
- Product overview
The ab100733 Mouse osteoprotegerin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse osteoprotegerin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated
streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of osteoprotegerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A . diluent buffer For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent. 1 x 15ml Detection Antibody Osteoprotegerin : biotinylated anti-mouse Osteoprotegerin 2 vials Osteoprotegerin Microplate (12 strips x 8 wells) coated with anti-mouse osteoprotegerin 1 unit Osteoprotegerin Standard Mouse (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
Contains 4 TNFR-Cys repeats.
modificationsN-glycosylated. Contains sialic acid residues.
The N-terminus is blocked.
- MGC29565 MGC93568OCIF
- OPGOsteoclastogenesis inhibitory factorOsteoprotegerinTNFRSF 11BTNFRSF11BTR 1TR1TR11B_HUMANTumor necrosis factor receptor superfamily member 11B
Our Abpromise guarantee covers the use of ab100733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Osteoprotegerin Mouse ELISA Kit (ab100733)
ab100733 has not yet been referenced specifically in any publications.