You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameOsteoprotegerin Mouse ELISA KitSee all Osteoprotegerin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 1 pg/ml
  • Range
    1.65 pg/ml - 1200 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 88.7 81% - 102%
    Serum 91.7 82% - 104%
    Plasma 81.9 65% - 89%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    The ab100733 Mouse osteoprotegerin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse osteoprotegerin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated

    streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of osteoprotegerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    200X HRP-Streptavidin Concentrate Item G 1 x 200µl
    20X Wash Buffer Concentrate Item B 1 x 25ml
    Assay Diluent A . diluent buffer For Standard/Sample (serum/plasma) diluent. 1 x 30ml
    Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent. 1 x 15ml
    Detection Antibody Osteoprotegerin : biotinylated anti-mouse Osteoprotegerin 2 vials
    Osteoprotegerin Microplate (12 strips x 8 wells) coated with anti-mouse osteoprotegerin 1 unit
    Osteoprotegerin Standard Mouse (recombinant) 2 vials
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • FunctionActs as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.
  • Tissue specificityHighly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.
  • Involvement in diseaseDefects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal.
  • Sequence similaritiesContains 2 death domains.
    Contains 4 TNFR-Cys repeats.
  • Post-translational
    modifications    N-glycosylated. Contains sialic acid residues.
    The N-terminus is blocked.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession O00300 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        MGC29565 MGC93568OCIF
        OPGOsteoclastogenesis inhibitory factorOsteoprotegerinTNFRSF 11BTNFRSF11BTR 1TR1TR11B_HUMANTumor necrosis factor receptor superfamily member 11B
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100733 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Osteoprotegerin Mouse ELISA Kit images

      • ELISA - Mouse Osteoprotegerin ELISA Kit (ab100733)
        ELISA - Mouse Osteoprotegerin ELISA Kit (ab100733)

      Protocols

      References for Osteoprotegerin Mouse ELISA Kit (ab100733)

      ab100733 has not yet been referenced specifically in any publications.

      Publishing research using ab100733 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100733 Submit a Question for ab100733

      Displaying 1 - 5 of 5 results for Abreviews and Q&A

      10
      Votes: Highest First

      Thank you for the reply to Olga, I will take over now. Did you also have OPG ELISA kit to offer?

      RANKL (ab100749) seems to be suitable, are you willing to quote in 25 kits? (or 25 plates if you have kits with more than one plate)

      I wil...

      Read More

      Thank you for your email.

      Yes, we do have OPG kits available. The catalogue number is ab100617 and ab100733, one is specific to human and other one to mouse.

      http://www.abcam.com/Osteoprotegerin-Human-ELISA-Kit-1-x-96-Well-Plate-ab100617...

      Read More


      According to our phone conversation, attached is my results. I really appreciate your help.
      Best Regards,

      Thank you for sending along the data.

      In both graphs the concentrations are listed in log scale but the ODs are shown in linear scale. The ODs should also be in log scale on the y axis.

      The OPG data looks as expected, where the concent...

      Read More

      Phone call enquiring as to whether the was buffer from the Osteoprotegerin Mouse ELISA Kit RANKL (ab100733) and mouse ELISA Kit (ab100749)

      Thank you for calling us on Friday.

      I can now confirm to you that the wash buffers of the two kits (ab100733 and ab100749) are interchangeable and if you want you could just make up one for one kit and use with both kits until you need to make u...

      Read More

      Hello,

      We currently use your RANKL and OPG ELISA kits for testing in mouse plasma (catalogue numbers ab100749 and ab100733).

      I was wondering if there is any cross-reactivity in human plasma.

      Thank you,

      Thank you for your inquiry.



      My colleague is out of office for a couple of days this week and he has asked me to look after his customers during his absence.



      Here is some information from the lab regarding these tw...

      Read More

      We currently use your RANKL and OPG ELISA kits for testing in mouse plasma (catalogue numbers ab100749 and ab100733).

      I was wondering if there is any cross-reactivity in human plasma.
      Thank you,

      Thank you for contacting us. I have been in contact with lab and now waiting for their reply. I will send you the requested info soon. Many thanks for having patience.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"