Overview

  • Product nameOsteoprotegerin Mouse ELISA KitSee all Osteoprotegerin kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    1.65 pg/ml - 1200 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 88.7 81% - 102%
    Serum 91.7 82% - 104%
    Plasma 81.9 65% - 89%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Osteoprotegerin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Osteoprotegerin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteoprotegerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionActs as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.
  • Tissue specificityHighly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.
  • Involvement in diseaseDefects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal.
  • Sequence similaritiesContains 2 death domains.
    Contains 4 TNFR-Cys repeats.
  • Post-translational
    modifications
    N-glycosylated. Contains sialic acid residues.
    The N-terminus is blocked.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession O00300 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • MGC29565
    • MGC93568
    • OCIF
    • OPG
    • Osteoclastogenesis inhibitory factor
    • Osteoprotegerin
    • TNFRSF 11B
    • TNFRSF11B
    • TR 1
    • TR1
    • TR11B_HUMAN
    • Tumor necrosis factor receptor superfamily member 11B
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Osteoprotegerin Mouse ELISA Kit images

  • Representative standard curve using ab100733

  • Representative standard curve using ab100733

Protocols

References for Osteoprotegerin Mouse ELISA Kit (ab100733)

ab100733 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.

Yes, we do have OPG kits available. The catalogue number is ab100617 and ab100733, one is specific to human and other one to mouse.

http://www.abcam.com/Osteoprotegerin-Human-ELISA-Kit-1-x-96-Well-Plate-ab100...

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Thank you for sending along the data.

In both graphs the concentrations are listed in log scale but the ODs are shown in linear scale. The ODs should also be in log scale on the y axis.

The OPG data looks as expected, where the conc...

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Thank you for calling us on Friday.

I can now confirm to you that the wash buffers of the two kits (ab100733 and ab100749) are interchangeable and if you want you could just make up one for one kit and use with both kits until you need to mak...

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Thank you for your inquiry.



My colleague is out of office for a couple of days this week and he has asked me to look after his customers during his absence.



Here is some information from the lab regarding these...

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Thank you for contacting us. I have been in contact with lab and now waiting for their reply. I will send you the requested info soon. Many thanks for having patience.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"