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Product Name
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Otoferlin antibody [13A9]
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [13A9] to Otoferlin
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Immunogen
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Tagged fusion protein, corresponding to amino acids 1-395 of Human Otoferlin.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody reacts specifically with human Otoferlin protein (220 kDa).
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100 - 1/500.
IHC-FoFr: 1/50 (PMID 19417007).
IP: 1/50 - 1/200.
WB: 1/200 - 1/1000. Predicted molecular weight: 227 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; single-pass type II membrane protein.
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Research areas
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Neuroscience >> Sensory System >> Auditory system
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs
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Relevance
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Otoferlin is a transmembrane protein with six C2 domains which locates to synaptic vesicles. It belongs to the family of ferlin C2 proteins which are implicated in membrane-membrane fusion events. Otoferlin is expressed in hair cells of the inner ear and in some brain regions. Mutations in the otoferlin gene are associated with the recessive prelingual deafness DFNB9.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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13A9
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Ascites
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Storage buffer
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Preservative: None
Constituents: Ascites
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Otoferlin antibody [13A9] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Otoferlin antibody [13A9] (ab53233)
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This product has been used in: (two most recent references)
Johnson SL et al. Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at auditory ribbon synapses. Nat Neurosci 13:45-52 (2010). IHC-Fr; Mouse, Rat. PubMed: 20010821
Heidrych P et al. Otoferlin interacts with myosin VI: implications for maintenance of the basolateral synaptic structure of the inner hair cell. Hum Mol Genet 18:2779-90 (2009). IHC-FoFr; Mouse, Rat. PubMed: 19417007
See all 3 publication references for this product.
If you publish research using ab53233 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Otoferlin
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Otoferlin antibody [13A9] - more information
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