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Product Name
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Otoferlin antibody [13A9]
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [13A9] to Otoferlin
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Immunogen
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Tagged fusion protein, corresponding to amino acids 1-395 of Human Otoferlin.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody reacts specifically with human Otoferlin protein (220 kDa).
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100 - 1/500.
IHC-FoFr: 1/50 (PMID 19417007).
IP: 1/50 - 1/200.
WB: 1/200 - 1/1000. Predicted molecular weight: 227 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; single-pass type II membrane protein.
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Research areas
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Neuroscience >> Sensory System >> Auditory system
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs
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Relevance
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Otoferlin might be involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion.
Mutations in the gene for Otoferlin are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has 3 C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has 6 C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multiple isoforms have been found for this gene.
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Otoferlin antibody [13A9] (ab53233) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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13A9
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Ascites
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Storage buffer
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Preservative: None
Constituents: Ascites
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Otoferlin antibody [13A9] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Otoferlin antibody [13A9] (ab53233)
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This product has been used in: (two most recent references)
Johnson SL et al. Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at auditory ribbon synapses. Nat Neurosci 13:45-52 (2010). IHC-Fr; Mouse, Rat. PubMed: 20010821
Heidrych P et al. Otoferlin interacts with myosin VI: implications for maintenance of the basolateral synaptic structure of the inner hair cell. Hum Mol Genet 18:2779-90 (2009). IHC-FoFr; Mouse, Rat. PubMed: 19417007
See all 3 publication references for this product.
If you publish research using ab53233 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Otoferlin
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Otoferlin antibody [13A9] - more information
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