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Western blot
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Immunocytochemistry/ Immunofluorescence - Otx2 antibody (ab21990)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Otx2 antibody - ChIP Grade (ab21990)
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Product Name
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Otx2 antibody - ChIP Grade
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See all Otx2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Otx2 - ChIP Grade
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Otx2.
(Note: the amino acid sequence is proprietar y)
(Pepti available as ab24347.)
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Reacts with
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Hu, Ms, Cow, Xl
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Predicted to react
(species key)
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Chk, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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ChIP, ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 18057103).
ICC/IF: Use at a concentration of 0.8 - 4 µg/ml.
IHC-Fr: Use at a concentration of 0.8 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Can be blocked with Otx2 peptide (ab24347).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Nervous system development
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Embryogenesis >> Axis formation and symmetry
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Stem Cells >> Lineage Markers >> Ectoderm
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Sensory System >> Visual system
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Relevance
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Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified.
Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.6 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Otx2 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Otx2 antibody - ChIP Grade images, references, products related to ab21990 and other tools.
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Otx2 antibody - ChIP Grade images:
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 Western blot
All lanes : Otx2 antibody - ChIP Grade (ab21990) at 1 µg/ml
Lane 1 : Y79 Lysate
Lane 2 : Y79 Lysate with Otx2 peptide (ab24347) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size : 37 kDa
Observed band size : 37 kDa
Additional bands at : 28 kDa (possible cleavage fragment,isoform,non-specific binding).
ab21990 recognised a band corresponding to the predicted size of Otx2 in Y79 cell lysate (lane 1, see arrow). The band was blocked using the immunizing peptide (lane 2). A less intense band was observed at ~28 kDa, which may be a cleavage product or an Otx2 isoform. Alternatively the ~28 kDa band may respresent non-specific binding by the antibody.
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Immunocytochemistry/ Immunofluorescence - Otx2 antibody (ab21990)
The image shows staining of Otx2 in human embryonic stem cells differentiated into neuroectoderm. As would be expected, staining is nuclear. L. Vallier, University of Cambridge
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Otx2 antibody - ChIP Grade (ab21990)
Mouse E6 embryos were sectioned and then fixed in paraformaldehyde. Antigen retrieval was performed using citric acid and the sections permeablized and blocked for 1 hour in serum. Embryos were stained with ab21990 (1/2500) diluted in 10% serum, 1% ovalbumin in PBSTween for 14 hours at 4°C. They were then washed and stained with a goat anti-rabbit biotin conjugated antibody. After using a biotinylated secondary, ABC and then DAB was used for color development.
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References for Otx2 antibody - ChIP Grade (ab21990)
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This product has been used in: (two most recent references)
Esumi N et al. BEST1 expression in the retinal pigment epithelium is modulated by OTX family members. Hum Mol Genet 18:128-41 (2009). WB, ChIP; Cow. PubMed: 18849347
Larder R & Mellon PL Otx2 Induction of the Gonadotropin-releasing Hormone Promoter Is Modulated by Direct Interactions with Grg Co-repressors. J Biol Chem 284:16966-78 (2009). WB, ChIP. PubMed: 19401468
See all 7 publication references for this product.
If you publish research using ab21990 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Otx2
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