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Western blot - Oxa1 antibody (ab21863)
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Product Name
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Oxa1 antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to Oxa1
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Immunogen
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Fusion protein: YVKSSDTVAR NSHIKPELDA LNNKLMSTTD LQQGQLVAMQ RKKLLSSHGI KNRWLAAPML QIPIALGFFN ALRHMANYPV DGFANQGVAW FTDLTQADPY corresponding to amino acids 147/246 of S. cerevisiae Oxa1.
BLAST 'YVKSSDTVAR NSHIKPELDA LNNKLMSTTD LQQGQLVAMQ RKKLLSSHGI KNRWLAAPML QIPIALGFFN ALRHMANYPV DGFANQGVAW FTDLTQADPY ' with  or 
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Reacts with
(species key)
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Sc
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 45 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
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Cellular localization
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Integral membrane protein. Mitochondria; inner membrane.
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Research areas
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Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Relevance
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Oxa1 is required for the insertion of integral membrane proteins into the mitochondrial inner membrane. It is essential for the activity and assembly of cytochrome c oxidase. It plays a central role in the translocation and export of the N-terminal part of the COX2 protein into the mitochondrial intermembrane space. It interacts with the large ribosome subunit of mitochondrial ribosome. It interacts directly with MRP20.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 856898 (Saccharomyces cerevisiae)
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| SwissProt | | P39952 (Saccharomyces cerevisiae)
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Constituents: 50% Glycerol Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Notes
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Produced from outbred CD1 mice
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Oxa1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Oxa1 antibody images, references, products related to ab21863 and other tools.
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Oxa1 antibody images:
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 Western blot - Oxa1 antibody (ab21863)
All lanes : Oxa1 antibody (ab21863) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 45 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
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Search PubMed (MEDLINE) for references to Oxa1
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Oxa1 antibody - more information
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Customer reviews (feedback) regarding Oxa1 antibody |
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Oxa1 antibody - related products:
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Oxa1 antibody - other tools:
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