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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-Oxa1 antibody
Description
Mouse polyclonal to Oxa1
Tested applications
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Fusion protein: YVKSSDTVARNSHIKPELDALNNKLMSTTDLQQGQLVAMQ RKKLLSSHGIKNRWLAAPMLQIPIALGFFNALRHMANYPV DGFANQGVAWFTDLTQADPY, corresponding to amino acids 147/246 of S. cerevisiae Oxa1.
YVKSSDTVAR NSHIKPELDA LNNKLMSTTD LQQGQLVAMQ RKKLLSSHGI KNRWLAAPML QIPIALGFFN ALRHMANYPV DGFANQGVAW FTDLTQADPY
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Western blot - Oxa1 antibody (ab21863)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 45 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Relevance
Oxa1 is required for the insertion of integral membrane proteins into the mitochondrial inner membrane. It is essential for the activity and assembly of cytochrome c oxidase. It plays a central role in the translocation and export of the N-terminal part of the COX2 protein into the mitochondrial intermembrane space. It interacts with the large ribosome subunit of mitochondrial ribosome. It interacts directly with MRP20.
Cellular localization
Integral membrane protein. Mitochondria; inner membrane.
Alternative names
- Cytochrome oxidase biogenesis protein OXA1 antibody
- Inner membrane protein OXA1 mitochondrial antibody
- Oxa1p antibody
- Oxidase assembly protein 1 antibody
Database links
- Entrez Gene: 856898 Saccharomyces cerevisiae
- SwissProt: P39952 Saccharomyces cerevisiae
Anti-Oxa1 antibody images:
Western blot - Oxa1 antibody (ab21863)
All lanes : Anti-Oxa1 antibody (ab21863) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 45 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
References for Anti-Oxa1 antibody (ab21863)
ab21863 has not yet been referenced specifically in any publications.
Publishing research using ab21863? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"