Anti-P Glycoprotein antibody [265/F4] (ab3083)
- Product nameAnti-P Glycoprotein antibody [265/F4]See all P Glycoprotein primary antibodies ...
- DescriptionMouse monoclonal [265/F4] to P Glycoprotein
- SpecificityWe have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
- Tested applicationsFlow Cyt, IHC-Fr, ICC/IF, WB more details
- Species reactivityReacts with: Hamster
Colchicine resistant viable Chinese Hamster Ovary.
- Positive controlDrug resistant Chinese Hamster Ovary (CHO) cells.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage buffer10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
- Concentration information loading...
- Clonality Monoclonal
- Clone number265/F4
- Light chain typekappa
- Research Areas
Our Abpromise guarantee covers the use of ab3083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt: Use at an assay dependent dilution.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution. Fix with acetone.|
|ICC/IF||ICC/IF: Use at an assay dependent concentration.|
|WB||WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa). 2 hours at RT.|
- FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
- Tissue specificityExpressed in liver, kidney, small intestine and brain.
- Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
- Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Cellular localizationMembrane.
- ABC20 antibodyABCB1 antibodyATP binding cassette sub family B MDR/TAP member 1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibodyATP-binding cassette sub-family B member 1 antibodyCD243 antibodyCD243 antigen antibodyCLCS antibodyColchicin sensitivity antibodyDoxorubicin resistance antibodyGP170 antibodyMDR 1 antibodyMDR1 antibodyMDR1_HUMAN antibodyMultidrug resistance 1 antibodyMultidrug resistance protein 1 antibodyP glycoprotein 1 antibodyP gp antibodyP-glycoprotein 1 antibodyPGY1 antibody
Anti-P Glycoprotein antibody [265/F4] images
All lanes : Anti-P Glycoprotein antibody [265/F4] (ab3083) at 1/2000 dilution
Lane 1 : Nuclear lysate prepared from human DU145 cells
Lane 2 : Nuclear lysate prepared from human DU145 cells
Lane 3 : Nuclear lysate prepared from human DU145 cells
Lane 4 : Nuclear lysate prepared from human DU145 cells
Lysates/proteins at 20 µg per lane.
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/10000 dilution
developed using the ECL technique
Predicted band size : 170 kDa
Exposure time : 30 seconds
Image courtesy of an anonymous Abreview.
References for Anti-P Glycoprotein antibody [265/F4] (ab3083)
This product has been referenced in:
- Lathan B et al. Immunological detection of Chinese hamster ovary cells expressing a multidrug resistance phenotype. Cancer Res 45:5064-9 (1985). Read more (PubMed: 2862990) »