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Anti-P Glycoprotein antibody [265/F4] (ab3083)

Abreviews (2)Q&A (7)Specific References (1)

Overview

  • Product nameAnti-P Glycoprotein antibody [265/F4]See all P Glycoprotein primary antibodies ...
  • Description
    Mouse monoclonal [265/F4] to P Glycoprotein
  • SpecificityWe have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsFlow Cyt, IHC-Fr, ICC/IF, WB more details
  • Species reactivity
    Reacts with: Hamster
  • Immunogen

    Colchicine resistant viable Chinese Hamster Ovary.

  • Positive controlDrug resistant Chinese Hamster Ovary (CHO) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab3083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: Use at an assay dependent dilution.
IHC-Fr IHC-Fr: Use at an assay dependent dilution. Fix with acetone.
ICC/IF ICC/IF: Use at an assay dependent concentration.
WB WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa). 2 hours at RT.

Target

  • FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
  • Tissue specificityExpressed in liver, kidney, small intestine and brain.
  • Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P08183 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
      ABC20 antibodyABCB1 antibodyATP binding cassette sub family B MDR/TAP member 1 antibody
      ATP binding cassette, sub family B (MDR/TAP), member 1 antibodyATP-binding cassette sub-family B member 1 antibodyCD243 antibodyCD243 antigen antibodyCLCS antibodyColchicin sensitivity antibodyDoxorubicin resistance antibodyGP170 antibodyMDR 1 antibodyMDR1 antibodyMDR1_HUMAN antibodyMultidrug resistance 1 antibodyMultidrug resistance protein 1 antibodyP glycoprotein 1 antibodyP gp antibodyP-glycoprotein 1 antibodyPGY1 antibody
    see all

Anti-P Glycoprotein antibody [265/F4] images

  • Western blot - Anti-P Glycoprotein antibody [265/F4] (ab3083)
    Western blot - Anti-P Glycoprotein antibody [265/F4] (ab3083)
    All lanes : Anti-P Glycoprotein antibody [265/F4] (ab3083) at 1/2000 dilution

    Lane 1 : Nuclear lysate prepared from human DU145 cells
    Lane 2 : Nuclear lysate prepared from human DU145 cells
    Lane 3 : Nuclear lysate prepared from human DU145 cells
    Lane 4 : Nuclear lysate prepared from human DU145 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/10000 dilution
    developed using the ECL technique

    Predicted band size : 170 kDa


    Exposure time : 30 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-P Glycoprotein antibody [265/F4] (ab3083)

This product has been referenced in:
  • Lathan B  et al. Immunological detection of Chinese hamster ovary cells expressing a multidrug resistance phenotype. Cancer Res 45:5064-9 (1985). Read more (PubMed: 2862990) »

See 1 Publication for this product

Publishing research using ab3083 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

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Displaying 1 - 9 of 9 results for Abreviews and Q&A

10
Votes: Highest First

Dear Abcam,Thanks for the information. Also i have few more doubts which i thought of asking you.   1) If i happen to use one of your primary antibody for p-gp, would i only see a band of around 140 kda and not the expected 170 kda? Reason i ask this is...

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In reviewing the information for human and mouse G-GP (NCBI Reference Sequence: NP_000918.2, NP_035206.2) it does seem that these have an expected size of ~140kDA before modification. If the sample is run in a reduced denatured form I would expect the ban...

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Hi, We have Anti-P Glycoprotein [265/F4] (ab3083) antibody in our lab and we are planning to use it during western blotting to identify the expressed P-gp in pig kidney epithelial cells. We would like to know the epitope sequence which this antibody ident...

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Thank you for your inquiry.

This product has been raised againstColchicine resistant viable Chinese Hamster Ovary tissue.Unfortunately, we have not mapped the epitope location for this particular antibody.

We aim to provide as mu...

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Hello fellows from abcam, My lab acquired the antibody for P-glycoprotein ab3083. I would like to perform a imunnohistochemistry with paraffin embeded slices in human material. Unfortunately, I did not find any information on its datasheet about buf...

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Thank you for contacting us.

We have not yet tested this product in IHC-P and so I am unable to provide spefic information for this product. I will attach our IHC-P protocol for your review however this has not been optimized for this product. ...

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Please can you supply antibody that could be used for Flow cytometry detection of any of these markers - MDR1, MRP1 and BCRP, and that would have concentration of at least 1 mg/ml.

thank you very much

best regards

Thank you for your enquiry and your interest in our products.

Here are a few antibodies against the following targets which have been tested and characterized for Flow cytometry. Please take a look at the on-line datasheets for further informati...

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Western blot abreview for Anti-P Glycoprotein antibody [265/F4]

 Excellent
Abreviews
Application Western blot
Sample Human Cell lysate - nuclear (DU145 cells)
Loading amount 20 µg
Specification DU145 cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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Verified customer

Submitted Nov 17 2011

Dear Sir/Madam, I was recently reading an article which uses one of your antibodies. I cannot seem to locate this product on your website and was wondering if you could point me in the right direction. The article states using: "a mouse-monoclonal a...

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Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the datas...

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Immunocytochemistry/ Immunofluorescence abreview for Anti-P Glycoprotein antibody [265/F4]

 Excellent
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (Liver cell line)
Specification Liver cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton X-100
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
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Username

Verified customer

Submitted Apr 16 2008

Thanks for your help. I have done some further investigation and think the best antibody to use is ab3364 as the data sheet gives me the amino acid sequence for the antigen and this is conserved in zebrafish. However, please can you check this antigen s...

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Thank you for getting back to us. Your enquiry has been passed onto me by my colleague in customer services. I can confirm that the epitope information detailed on our antibody datasheet is correct. The antibody has been shown to work in Mouse, Rat an...

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BATCH NUMBER 215820 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No bands or very faint bands achieved after multiple optimisation attempts and at 1:10 dilution of antibody SAMPLE mouse tumour lysate, murine and human...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. P Glycoprotein antibody [265/F4] (ab3083) has been shown to reacts with Hamster P Glycoprotein and not against the human homolog of this protein....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"