Anti-P Glycoprotein antibody [F4] - BSA and Azide free (ab80594)

Overview

• Product nameAnti-P Glycoprotein antibody [F4] - BSA and Azide freeSee all P Glycoprotein primary antibodies ...
• Description
  Mouse monoclonal [F4] to P Glycoprotein - BSA and Azide free
• Specificityab80594 detects soluble P glycoprotein in the cultured media of viable adriamycin-resistant cells, whereas those of the drug-sensitive parent cells contain no detectable level of soluble P glycoprotein. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
• Tested applicationsIHC-P, WB, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human, Chinese Hamster
  
• Immunogen

  Human and hamster drug-resistant viable cells followed by crude plasma membranes.

• EpitopeExtracellular amino terminal half. This epitope is resistant to formalin fixation and periodate oxidation.
• Positive controlDrug resistant cells. Human kidney or breast carcinoma.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 10mM PBS, pH 7.4
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberF4
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Stem Cells
  • Hematopoietic Progenitors
  • Surface Molecules

  • Cancer
  • Drug resistance
  • P glycoproteins

  • Signal Transduction
  • Metabolism
  • Plasma Membrane
  • Channels

Applications

Target

• FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
• Tissue specificityExpressed in liver, kidney, small intestine and brain.
• Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
• Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
  Contains 2 ABC transmembrane type-1 domains.
  Contains 2 ABC transporter domains.
• Cellular localizationMembrane.

Target information above from: UniProt accession P08183 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5243 Human
  • Omim: 171050 Human
  • SwissProt: P08183 Human
  • Unigene: 489033 Human

• Alternative names
  ABC20 antibodyABCB1 antibodyATP binding cassette sub family B MDR/TAP member 1 antibody

  ATP binding cassette, sub family B (MDR/TAP), member 1 antibodyATP-binding cassette sub-family B member 1 antibodyCD243 antibodyCD243 antigen antibodyCLCS antibodyColchicin sensitivity antibodyDoxorubicin resistance antibodyGP170 antibodyMDR 1 antibodyMDR1 antibodyMDR1_HUMAN antibodyMultidrug resistance 1 antibodyMultidrug resistance protein 1 antibodyP glycoprotein 1 antibodyP gp antibodyP-glycoprotein 1 antibodyPGY1 antibody

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