Anti-P Glycoprotein antibody [F4] - BSA and Azide free (ab80594)
- Product nameAnti-P Glycoprotein antibody [F4] - BSA and Azide freeSee all P Glycoprotein primary antibodies ...
- DescriptionMouse monoclonal [F4] to P Glycoprotein - BSA and Azide free
- Specificityab80594 detects soluble P glycoprotein in the cultured media of viable adriamycin-resistant cells, whereas those of the drug-sensitive parent cells contain no detectable level of soluble P glycoprotein. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
- Tested applicationsIHC-P, WB, Flow Cyt, ICC/IF more details
- Species reactivityReacts with: Human, Chinese Hamster
Human and hamster drug-resistant viable cells followed by crude plasma membranes.
- EpitopeExtracellular amino terminal half. This epitope is resistant to formalin fixation and periodate oxidation.
- Positive controlDrug resistant cells. Human kidney or breast carcinoma.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 10mM PBS, pH 7.4
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberF4
- Research Areas
Our Abpromise guarantee covers the use of ab80594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 141 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
- Tissue specificityExpressed in liver, kidney, small intestine and brain.
- Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
- Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Cellular localizationMembrane.
- ABC20 antibodyABCB1 antibodyATP binding cassette sub family B MDR/TAP member 1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibodyATP-binding cassette sub-family B member 1 antibodyCD243 antibodyCD243 antigen antibodyCLCS antibodyColchicin sensitivity antibodyDoxorubicin resistance antibodyGP170 antibodyMDR 1 antibodyMDR1 antibodyMDR1_HUMAN antibodyMultidrug resistance 1 antibodyMultidrug resistance protein 1 antibodyP glycoprotein 1 antibodyP gp antibodyP-glycoprotein 1 antibodyPGY1 antibody
References for Anti-P Glycoprotein antibody [F4] - BSA and Azide free (ab80594)
This product has been referenced in:
- Chu TM et al. Detection of soluble P-glycoprotein in culture media and extracellular fluids. Biochem Biophys Res Commun 203:506-12 (1994). WB . Read more (PubMed: 7915520) »
- Chu TM et al. Characterization of a new monoclonal antibody F4 detecting cell surface epitope and P-glycoprotein in drug-resistant human tumor cell lines. Hybridoma 12:417-29 (1993). WB, ICC/IF ; Chinese Hamster . Read more (PubMed: 7503940) »