Anti-P Glycoprotein antibody [F4] - BSA and Azide free (ab80594)

Overview

  • Product nameAnti-P Glycoprotein antibody [F4] - BSA and Azide freeSee all P Glycoprotein primary antibodies ...
  • Description
    Mouse monoclonal [F4] to P Glycoprotein - BSA and Azide free
  • Specificityab80594 detects soluble P glycoprotein in the cultured media of viable adriamycin-resistant cells, whereas those of the drug-sensitive parent cells contain no detectable level of soluble P glycoprotein. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsIHC-P, WB, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Human, Chinese Hamster
  • Immunogen

    Human and hamster drug-resistant viable cells followed by crude plasma membranes.

  • EpitopeExtracellular amino terminal half. This epitope is resistant to formalin fixation and periodate oxidation.
  • Positive control
    • Drug resistant cells. Human kidney or breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab80594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
WB
Flow Cyt
ICC/IF
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 141 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
    • Tissue specificityExpressed in liver, kidney, small intestine and brain.
    • Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
    • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • Cellular localizationMembrane.
    • Target information above from: UniProt accession P08183 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • ABC20 antibody
      • ABCB1 antibody
      • ATP binding cassette sub family B MDR/TAP member 1 antibody
      • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
      • ATP-binding cassette sub-family B member 1 antibody
      • CD243 antibody
      • CD243 antigen antibody
      • CLCS antibody
      • Colchicin sensitivity antibody
      • Doxorubicin resistance antibody
      • GP170 antibody
      • MDR 1 antibody
      • MDR1 antibody
      • MDR1_HUMAN antibody
      • Multidrug resistance 1 antibody
      • Multidrug resistance protein 1 antibody
      • P glycoprotein 1 antibody
      • P gp antibody
      • P-glycoprotein 1 antibody
      • PGY1 antibody
      see all

    References for Anti-P Glycoprotein antibody [F4] - BSA and Azide free (ab80594)

    This product has been referenced in:
    • Chu TM  et al. Detection of soluble P-glycoprotein in culture media and extracellular fluids. Biochem Biophys Res Commun 203:506-12 (1994). WB . Read more (PubMed: 7915520) »
    • Chu TM  et al. Characterization of a new monoclonal antibody F4 detecting cell surface epitope and P-glycoprotein in drug-resistant human tumor cell lines. Hybridoma 12:417-29 (1993). WB, ICC/IF ; Chinese Hamster . Read more (PubMed: 7503940) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us.

    Below is a link to our thorough western blot guide, which includes tips on sample preparation, running, and transfering large proteins.

    http://www.abcam.com/ps/pdf/protocols/WB-beginner.pdf

    For yo...

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    I can confirm that it should not be necessary to prepare a membrane fraction in order to detect this protein.

    In case you are experiencing high background or to enrich the protein when low signal occurs, I would recommend preparing ...

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    Thank you for passing on that reply.

    I am sorry to hear that the customer has still not observed the expected signal with the anti-P Glycoprotein antibody (ab3366).

    As I mentioned in my previous email, I would be happy to provide the ...

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    Thank you for your enquiry.

    I can confirm that the epitope of ab80594 Anti-P Glycoprotein antibody [F4] is within the extracellular amino terminal end of the protein.

    I hope this will be helpful. If you have any further questions,...

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    Thank you for your enquiry and your interest in our products.

    Here are a few antibodies against the following targets which have been tested and characterized for Flow cytometry. Please take a look at the on-line datasheets for further inform...

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    Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the da...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"