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Anti-P Glycoprotein antibody [UIC2] (FITC) (ab66250)


  • Product nameAnti-P Glycoprotein antibody [UIC2] (FITC)
    See all P Glycoprotein primary antibodies
  • Description
    Mouse monoclonal [UIC2] to P Glycoprotein (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab66250 specifically recognises an extracellular conformational epitope of P Glycoprotein.
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human PrimatesDoes not react with: Mouse, Rat
  • Immunogen

    Mouse Balb/c 3T3 fibroblasts transfected with human P Glycoprotein cDNA

  • General notesThis product is photosensitive and should be protected from light.

    Clone UIC2 does not crossreact with mitochondrial pyruvate carboxylase.



Our Abpromise guarantee covers the use of ab66250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. (in 100µl). Clone UIC2 can also be used in a shift assay to selectively demonstrate the expression and functional activity of P Glycoprotein in a target cell.

ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
  • Tissue specificityExpressed in liver, kidney, small intestine and brain.
  • Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC20 antibody
    • ABCB1 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
    • ATP-binding cassette sub-family B member 1 antibody
    • CD243 antibody
    • CLCS antibody
    • Colchicin sensitivity antibody
    • Doxorubicin resistance antibody
    • GP170 antibody
    • MDR1 antibody
    • MDR1_HUMAN antibody
    • Multidrug resistance 1 antibody
    • Multidrug resistance protein 1 antibody
    • P glycoprotein 1 antibody
    • P gp antibody
    • P-glycoprotein 1 antibody
    • PGY1 antibody
    see all

References for Anti-P Glycoprotein antibody [UIC2] (FITC) (ab66250)

This product has been referenced in:
  • Silva R  et al. P-glycoprotein induction in Caco-2 cells by newly synthetized thioxanthones prevents paraquat cytotoxicity. Arch Toxicol N/A:N/A (2014). Read more (PubMed: 25234084) »
  • Vilas-Boas V  et al. Development of novel rifampicin-derived P-glycoprotein activators/inducers. synthesis, in silico analysis and application in the RBE4 cell model, using paraquat as substrate. PLoS One 8:e74425 (2013). Read more (PubMed: 23991219) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us.

Lab has confirmed that they have not specifically determined what class of light chain this antibody possesses.

We apologise but we currently do not have that information available.

We have added ab166957 as Mouse monoclonal [UIC2] to P Glycoprotein - unconjugated form to our catalog.

I can confirm that I have sent an email to my colleague in lab.


Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (melanoma cell line)
Specification melanoma cell line
Preparation Cell harvesting/tissue preparation method: cell scraping
Sample buffer: PBS
Fixation no fixation
Permeabilization No
Gating Strategy Forward/Side scatter were used to eliminate cellular debris.

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Submitted Oct 30 2009