Anti-P Glycoprotein antibody [UIC2] (FITC) (ab66250)
- Product nameAnti-P Glycoprotein antibody [UIC2] (FITC)See all P Glycoprotein primary antibodies ...
- DescriptionMouse monoclonal [UIC2] to P Glycoprotein (FITC)
- ConjugationFITC. Ex: 493nm, Em: 528nm
- Specificityab66250 specifically recognises an extracellular conformational epitope of P Glycoprotein.
- Tested applicationsFlow Cyt more details
- Species reactivityReacts with: Human
Predicted to work with: Non Human Primates
Does not react withMouse, Rat
Mouse Balb/c 3T3 fibroblasts transfected with human P Glycoprotein cDNA
- General notesThis product is photosensitive and should be protected from light.
Clone UIC2 does not crossreact with mitochondrial pyruvate carboxylase.
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
- Concentration information loading...
- PurityProtein G purified
- Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Primary antibody notes Clone UIC2 does not crossreact with mitochondrial pyruvate carboxylase.
- Clonality Monoclonal
- Clone numberUIC2
- Research Areas
Our Abpromise guarantee covers the use of ab66250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt: Use 10µl for 106 cells. (in 100µl). Clone UIC2 can also be used in a shift assay to selectively demonstrate the expression and functional activity of P Glycoprotein in a target cell.|
- FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
- Tissue specificityExpressed in liver, kidney, small intestine and brain.
- Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
- Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Cellular localizationMembrane.
- ABC20 antibody
- ABCB1 antibody
- ATP binding cassette sub family B MDR/TAP member 1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
- ATP-binding cassette sub-family B member 1 antibody
- CD243 antibody
- CD243 antigen antibody
- CLCS antibody
- Colchicin sensitivity antibody
- Doxorubicin resistance antibody
- GP170 antibody
- MDR 1 antibody
- MDR1 antibody
- MDR1_HUMAN antibody
- Multidrug resistance 1 antibody
- Multidrug resistance protein 1 antibody
- P glycoprotein 1 antibody
- P gp antibody
- P-glycoprotein 1 antibody
- PGY1 antibody
References for Anti-P Glycoprotein antibody [UIC2] (FITC) (ab66250)
This product has been referenced in:
- Vilas-Boas V et al. Development of novel rifampicin-derived P-glycoprotein activators/inducers. synthesis, in silico analysis and application in the RBE4 cell model, using paraquat as substrate. PLoS One 8:e74425 (2013). Read more (PubMed: 23991219) »
- Boyerinas B et al. Let-7 modulates acquired resistance of ovarian cancer to Taxanes via IMP-1-mediated stabilization of multidrug resistance 1. Int J Cancer 130:1787-97 (2012). Read more (PubMed: 21618519) »