Anti-P Glycoprotein antibody [UIC2] (FITC) (ab66250)

Overview

  • Product nameAnti-P Glycoprotein antibody [UIC2] (FITC)See all P Glycoprotein primary antibodies ...
  • Description
    Mouse monoclonal [UIC2] to P Glycoprotein (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab66250 specifically recognises an extracellular conformational epitope of P Glycoprotein.
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates

    Does not react with

    Mouse, Rat
  • Immunogen

    Mouse Balb/c 3T3 fibroblasts transfected with human P Glycoprotein cDNA

  • General notesThis product is photosensitive and should be protected from light.


    Clone UIC2 does not crossreact with mitochondrial pyruvate carboxylase.

Properties

Applications

Our Abpromise guarantee covers the use of ab66250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. (in 100µl). Clone UIC2 can also be used in a shift assay to selectively demonstrate the expression and functional activity of P Glycoprotein in a target cell.

Target

  • FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
  • Tissue specificityExpressed in liver, kidney, small intestine and brain.
  • Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC20 antibody
    • ABCB1 antibody
    • ATP binding cassette sub family B MDR/TAP member 1 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
    • ATP-binding cassette sub-family B member 1 antibody
    • CD243 antibody
    • CD243 antigen antibody
    • CLCS antibody
    • Colchicin sensitivity antibody
    • Doxorubicin resistance antibody
    • GP170 antibody
    • MDR 1 antibody
    • MDR1 antibody
    • MDR1_HUMAN antibody
    • Multidrug resistance 1 antibody
    • Multidrug resistance protein 1 antibody
    • P glycoprotein 1 antibody
    • P gp antibody
    • P-glycoprotein 1 antibody
    • PGY1 antibody
    see all

References for Anti-P Glycoprotein antibody [UIC2] (FITC) (ab66250)

This product has been referenced in:
  • Vilas-Boas V  et al. Development of novel rifampicin-derived P-glycoprotein activators/inducers. synthesis, in silico analysis and application in the RBE4 cell model, using paraquat as substrate. PLoS One 8:e74425 (2013). Read more (PubMed: 23991219) »
  • Boyerinas B  et al. Let-7 modulates acquired resistance of ovarian cancer to Taxanes via IMP-1-mediated stabilization of multidrug resistance 1. Int J Cancer 130:1787-97 (2012). Read more (PubMed: 21618519) »

See all 4 Publications for this product

Product Wall


We have added ab166957 as Mouse monoclonal [UIC2] to P Glycoprotein - unconjugated form to our catalog.

http://www.abcam.com/index.html?datasheet=166957

Thank you for contacting us.

Lab has confirmed that they have not specifically determined what class of light chain this antibody possesses.

We apologise but we currently do not have that information available.

I can confirm that I have sent an email to my colleague in lab.

Thanks!

Application Flow Cytometry
Sample Human Cell (melanoma cell line)
Specification melanoma cell line
Preparation Cell harvesting/tissue preparation method: cell scraping
Sample buffer: PBS
Fixation no fixation
Permeabilization No
Gating Strategy Forward/Side scatter were used to eliminate cellular debris.
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Submitted Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"