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Anti-P Glycoprotein antibody [UIC2] (Phycoerythrin) (ab93590)

Submit an Abreview Q&A (4)Specific References (2)

Overview

  • Product nameAnti-P Glycoprotein antibody [UIC2] (Phycoerythrin)See all P Glycoprotein primary antibodies ...
  • Description
    Mouse monoclonal [UIC2] to P Glycoprotein (Phycoerythrin)
  • ConjugationPhycoerythrin
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mouse Balb/c 3T3 fibroblasts transfected with Human P Glycoprotein cDNA

  • Positive control8226 DOX40 and 8226/S cell lines

Properties

Applications

Our Abpromise guarantee covers the use of ab93590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
  • Application notesFlow Cyt: Use 5µl/test for 105-8 cells in 100ul.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
    • Tissue specificityExpressed in liver, kidney, small intestine and brain.
    • Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
    • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • Cellular localizationMembrane.

      • Target information above from: UniProt accession P08183 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          ABC20 antibodyABCB1 antibodyATP binding cassette, sub family B (MDR/TAP), member 1 antibody
          ATP-binding cassette sub-family B member 1 antibodyCD243 antibodyCLCS antibodyColchicin sensitivity antibodyDoxorubicin resistance antibodyGP170 antibodyMDR1 antibodyMDR1_HUMAN antibodyMultidrug resistance 1 antibodyMultidrug resistance protein 1 antibodyP glycoprotein 1 antibodyP gp antibodyP-glycoprotein 1 antibodyPGY1 antibody
        see all

      Anti-P Glycoprotein antibody [UIC2] (Phycoerythrin) images

      • Flow Cytometry - P Glycoprotein antibody [UIC2] (Phycoerythrin) (ab93590)
        Flow Cytometry - P Glycoprotein antibody [UIC2] (Phycoerythrin) (ab93590)
        Staining of the 8226/S (blue histogram) and 8226 DOX40 (purple histogram) cell lines with PE Mouse IgG2a, K Isotype Control (left) or ab93590 (right). Total viable cells were used for analysis.

      References for Anti-P Glycoprotein antibody [UIC2] (Phycoerythrin) (ab93590)

      This product has been referenced in:
      • Chaudhary PM  et al. Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes. Blood 80:2735-9 (1992). Read more (PubMed: 1360267) »
      • Mechetner EB & Roninson IB Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. Proc Natl Acad Sci U S A 89:5824-8 (1992). Read more (PubMed: 1352877) »

      See all 2 Publications for this product

      Publishing research using ab93590 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab93590 Submit a Question for ab93590

      Displaying 1 - 4 of 4 results for Abreviews and Q&A

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      Votes: Highest First

      Inquiry: I am going to buy the following antibody from Abcam Anti-P Glycoprotein antibody [UIC2] (Phycoerythrin) (ab93590). It states on the data sheet that the isotype control used for this is a mouse IgG2a kappa but the only one i can find on your site ...

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      Thank you for contacting us.

      Please buy ab91363.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Hi Tech team, how are you today?



      We have a customer who wants to know whether ab93590 and ab1282 can be used to detect Pgp on cancer cells by flow cytometry.



      Looking forward to your response soon.

      ...

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      Thank you for your inquiry.

      I am happy to confirm that ab93590 (the isotype control for it (ab1282) will not bind anything specifically, but mark the background staining) is suitable for flow cytometry. It will recognise P-glycoprotein 1 (Pgp) i...

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      Dear Sir/Madam,

      I would like to buy an antibody against to P-glycoprotein to detect over-expressing of P-gp on cancer cells using flow cytometry. Could you please let me know these below two products from your company are the right choice for m...

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      Thank you for your enquiry and your interest.

      I can confirm that ab93590has been testedin Flow cytometry and ab1282 can be used as isotype control.

      We also have other primary antibodies against P Glycoprotein, here are some non-conjuga...

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      phone call: concentration and indiciations for dilution

      Merci pour votre enquête ainsi que pour votre patience pendant que je contactais le laboratoire. Le laboratoire m'a pu répondre que la concentration de cet anticorps est d'environ 0.5ug par 5ul. Donc ceci fait 0.1mg/ml. Le laboratoire à testé 5ul de cet a...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"