Anti-P Glycoprotein antibody [UIC2] (Phycoerythrin) (ab93590)

Overview

  • Product nameAnti-P Glycoprotein antibody [UIC2] (Phycoerythrin)See all P Glycoprotein primary antibodies ...
  • Description
    Mouse monoclonal [UIC2] to P Glycoprotein (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mouse Balb/c 3T3 fibroblasts transfected with Human P Glycoprotein cDNA

  • Positive control
    • 8226 DOX40 and 8226/S cell lines
  • General notes


    ab93590 has been reported to inhibit MDR1-mediated efflux.

Properties

Applications

Our Abpromise guarantee covers the use of ab93590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 5µl/test for 105-8 cells in 100ul.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
    • Tissue specificityExpressed in liver, kidney, small intestine and brain.
    • Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
    • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABC20 antibody
      • ABCB1 antibody
      • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
      • ATP-binding cassette sub-family B member 1 antibody
      • CD243 antibody
      • CLCS antibody
      • Colchicin sensitivity antibody
      • Doxorubicin resistance antibody
      • GP170 antibody
      • MDR1 antibody
      • MDR1_HUMAN antibody
      • Multidrug resistance 1 antibody
      • Multidrug resistance protein 1 antibody
      • P glycoprotein 1 antibody
      • P gp antibody
      • P-glycoprotein 1 antibody
      • PGY1 antibody
      see all

    Anti-P Glycoprotein antibody [UIC2] (Phycoerythrin) images

    • Staining of the 8226/S (blue histogram) and 8226 DOX40 (purple histogram) cell lines with PE Mouse IgG2a, K Isotype Control (left) or ab93590 (right). Total viable cells were used for analysis.

    References for Anti-P Glycoprotein antibody [UIC2] (Phycoerythrin) (ab93590)

    This product has been referenced in:
    • Huang XC  et al. Parguerenes: Marine red alga bromoditerpenes as inhibitors of P-glycoprotein (ABCB1) in multidrug resistant human cancer cells. Biochem Pharmacol 85:1257-68 (2013). Read more (PubMed: 23415901) »
    • Chaudhary PM  et al. Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes. Blood 80:2735-9 (1992). Read more (PubMed: 1360267) »

    See all 3 Publications for this product

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    Please buy ab91363.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Thank you for your inquiry.

    I am happy to confirm that ab93590 (the isotype control for it (ab1282) will not bind anything specifically, but mark the background staining) is suitable for flow cytometry. It will recognise P-glycoprotein 1 (Pgp...

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    Thank you for your enquiry and your interest.

    I can confirm that ab93590has been testedin Flow cytometry and ab1282 can be used as isotype control.

    We also have other primary antibodies against P Glycoprotein, here are some non-conj...

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    Merci pour votre enquête ainsi que pour votre patience pendant que je contactais le laboratoire. Le laboratoire m'a pu répondre que la concentration de cet anticorps est d'environ 0.5ug par 5ul. Donc ceci fait 0.1mg/ml. Le laboratoire à testé 5ul de ce...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"