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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> P2X Receptors
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab76893? Please let us know so that we can cite the reference in this datasheet
ab76893 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - P2RX4 antibody (ab76893)
All lanes : Anti-P2RX4 antibody (ab76893) at 1 µg/ml
Lane 1 :
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
P2RX4 contains a number of post-translational modification sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, the 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to this target.
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