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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> P2X Receptors
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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - P2RX4 antibody (ab76893)
All lanes : Anti-P2RX4 antibody (ab76893) at 1 µg/ml
Lane 1 :
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
P2RX4 contains a number of post-translational modification sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, the 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to this target.
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