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Western blot - P2RX4 antibody (ab76893)
(enlarge)
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Product Name
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P2RX4 antibody
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See all P2RX4 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to P2RX4
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human P2RX4.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
Human Brain Tissue; SHSY-5Y Whole Cell; SK N BE Whole Cell; SK N SH Whole Cell Lysate
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Cellular localization
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Membrane; multi-pass membrane protein.
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Research areas
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Cardiovascular >> Blood >> Blood Pressure regulation
Cell Biology >> Other Antibodies >> Other Antibodies
Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> P2X Receptors
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Relevance
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ATP exerts its neuromodulatory effects via activation of purinergic receptors. Currently, 14 purinergic receptors are known and can be split into two classes: P2X and P2Y, with each class containing seven members. The P2X receptor subunits (P2X1-P2X7) can form either homomultimers or heteromultimers which then act as ligand-gated cation channels. P2X receptors are differentially distributed throughout the adrenal gland, heart and CNS.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.900 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this P2RX4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for P2RX4 antibody images, references, products related to ab76893 and other tools.
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P2RX4 antibody images:
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 Western blot - P2RX4 antibody (ab76893)
All lanes : P2RX4 antibody (ab76893) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
P2RX4 contains a number of post-translational modification sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, the 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to this target.
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Search PubMed (MEDLINE) for references to P2RX4
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P2RX4 antibody - more information
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Customer reviews (feedback) regarding P2RX4 antibody |
Customer FAQs regarding P2RX4 antibody |
Protocols for P2RX4 antibody |
Price and availability of products related to P2RX4 antibody |
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P2RX4 antibody - related products:
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P2RX4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about P2RX4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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