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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> P2X Receptors
Overview
Product name
Anti-P2RX4 antibody
See all P2RX4 products (5) ...
Description
Rabbit polyclonal to P2RX4
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human P2RX4.
(Peptide available as ab89385.)
Positive control
This antibody gave a positive signal in the following lysates: Human Brain Tissue; SHSY-5Y Whole Cell; SK N BE Whole Cell; SK N SH Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Blood Pressure regulation
Cell Biology >> Other Antibodies >> Other Antibodies
Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> P2X Receptors
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Western blot - P2RX4 antibody (ab76893)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 75 kDa (predicted molecular weight: 43 kDa).
Target
Function
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Sequence similarities
Belongs to the P2X receptor family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ99571
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI504491 antibody
- wu:fi03f02 antibody
- ATP gated cation channel protein antibody
see all
Database links
- Entrez Gene: 5025 Human
- Omim: 600846 Human
- SwissProt: Q99571 Human
- Unigene: 321709 Human
Anti-P2RX4 antibody images:
Western blot - P2RX4 antibody (ab76893)
All lanes : Anti-P2RX4 antibody (ab76893) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
P2RX4 contains a number of post-translational modification sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, the 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to this target.
References for Anti-P2RX4 antibody (ab76893)
ab76893 has not yet been referenced specifically in any publications.
Publishing research using ab76893? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"