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Anti-P2RX4 antibody (ab82329)

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Overview

Product name

Anti-P2RX4 antibody
See all P2RX4 products (5) ...

Description

Rabbit polyclonal to P2RX4

Tested applications

ICC/IF, WB, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide from the cytoplasmic domain of Human P2RX4 conjugated to an immunogenic carrier protein. Peptide available as ab97902

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence-P2RX4 antibody(ab82329)Immunocytochemistry/ Immunofluorescence-P2RX4 antibody(ab82329) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab82329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 1 µg/ml

  • ShowHide

    WB

     WB: 1/300 - 1/2000.Predi...Read more →

    WB: 1/300 - 1/2000.Predicted molecular weight: 43 kDa.(This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)

  • IHC-P

     IHC-P: 1/300 - 1/2000.

    IHC-P: 1/300 - 1/2000.

  • IHC-Fr

     IHC-Fr: 1/300 - 1/2000.

    IHC-Fr: 1/300 - 1/2000.

Target

Function

Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.

Sequence similarities

Belongs to the P2X receptor family.

Cellular localization

Membrane.

Target information above from: UniProt accessionQ99571 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AI504491 antibody
  • wu:fi03f02 antibody
  • ATP gated cation channel protein antibody
  • ATP receptor antibody
  • AW555605 antibody
  • D5Ertd444e antibody
  • fi03f02 antibody
  • MGC143034 antibody
  • p2rx4 antibody
  • P2RX4_HUMAN antibody
  • P2X purinoceptor 4 antibody
  • P2X receptor subunit 4 antibody
  • P2X4 antibody
  • P2X4R antibody
  • Purinergic receptor antibody
  • Purinergic receptor P2X ligand gated ion channel 4 antibody
  • Purinergic receptor P2X4 antibody
  • Purinoceptor P2X4 antibody
see all

Anti-P2RX4 antibody images:

  Immunocytochemistry/ Immunofluorescence-P2RX4 antibody(ab82329)

Immunocytochemistry/ Immunofluorescence-P2RX4 antibody(ab82329)

ICC/IF image of ab82329 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82329, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-P2RX4 antibody (ab82329)

This product has been referenced in:

  • Wu Tet al. Functional expression of P2X4 receptor in capillary endothelial cells of the cochlear spiral ligament and its role in regulating the capillary diameter. Am J Physiol Heart Circ Physiol 301:H69-78 (2011). IHC-FrFl; Guinea pig.Read more (PubMed: 21460192) »

See 1 publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"