Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> P2X Receptors
Overview
Product name
Anti-P2RX4 antibody
See all P2RX4 products (5) ...
Description
Rabbit polyclonal to P2RX4
Tested applications
ICC/IF, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide from the cytoplasmic domain of Human P2RX4 conjugated to an immunogenic carrier protein. Peptide available as ab97902
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Blood Pressure regulation
Cell Biology >> Other Antibodies >> Other Antibodies
Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> P2X Receptors
-
Immunocytochemistry/ Immunofluorescence-P2RX4 antibody(ab82329)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/300 - 1/2000.Predicted molecular weight: 43 kDa.(This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)
IHC-P
IHC-P: 1/300 - 1/2000.IHC-P: 1/300 - 1/2000.
IHC-Fr
IHC-Fr: 1/300 - 1/2000.IHC-Fr: 1/300 - 1/2000.
Target
Function
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Sequence similarities
Belongs to the P2X receptor family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ99571
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI504491 antibody
- wu:fi03f02 antibody
- ATP gated cation channel protein antibody
see all
Database links
- Entrez Gene: 5025 Human
- Entrez Gene: 18438 Mouse
- Entrez Gene: 29659 Rat
- Omim: 600846 Human
- SwissProt: Q99571 Human
- SwissProt: Q9JJX6 Mouse
- SwissProt: P51577 Rat
- Unigene: 321709 Human
see all
Anti-P2RX4 antibody images:
Immunocytochemistry/ Immunofluorescence-P2RX4 antibody(ab82329)
ICC/IF image of ab82329 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82329, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-P2RX4 antibody (ab82329)
This product has been referenced in:
- Wu Tet al. Functional expression of P2X4 receptor in capillary endothelial cells of the cochlear spiral ligament and its role in regulating the capillary diameter. Am J Physiol Heart Circ Physiol 301:H69-78 (2011). IHC-FrFl; Guinea pig.Read more (PubMed: 21460192) »
See 1 publication for this product
Publishing research using ab82329? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"