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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> P2X Receptors
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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab10269? Please let us know so that we can cite the reference in this datasheet
ab10269 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (PFA perfusion fixed frozen sections) - P2X3 antibody (ab10269)
Image A: ab10248, at a dilution of 1/1000, staining P2X3 (green) in rat dorsal root ganglia sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Other structures visualized with anti-ASIC3 (red). Image B: Structures in rat dorsal root ganglia sections visualized with anti-ASIC3 (red) and anti-TRPV1 (green). Double-labeled cells are yellow or orange.
Immunocytochemistry/ Immunofluorescence-P2X3 antibody(ab10269)
ICC/IF image of ab10269 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10269, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
P2X3 antibody for WB in Rat (10269)
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