Recombinant Anti-P2Y12 antibody [EPR18611] (ab184411)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18611] to P2Y12
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-P2Y12 antibody [EPR18611]
See all P2Y12 primary antibodies -
Description
Rabbit monoclonal [EPR18611] to P2Y12 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; mouse and rat brain tissue lysates. IP: Rat brain whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18611 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/80.
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WB |
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).
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Notes |
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IP
1/80. |
WB
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation. -
Tissue specificity
Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain. -
Involvement in disease
Defects in P2RY12 are the cause of P2RY12 deficiency (P2RY12D) [MIM:609821]. It is a condition characterized by severe impairment of platelet response to ADP and abnormal bleeding marked by excessive posttraumatic and postsurgical blood loss. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 70839 Mouse
- Entrez Gene: 64803 Rat
- SwissProt: Q9CPV9 Mouse
- SwissProt: Q9EPX4 Rat
- Unigene: 422675 Mouse
- Unigene: 64592 Rat
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Alternative names
- ADP glucose receptor antibody
- ADP-glucose receptor antibody
- ADPG R antibody
see all
Images
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P2Y12 was immunoprecipitated from 1mg of Rat brain whole cell lysate with ab184411 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab184411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Rat brain whole cell lysate 10µg (Input).
Lane 2: ab184411 IP in Rat brain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184411 in Rat brain whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/2000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/2000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (11)
ab184411 has been referenced in 11 publications.
- Sun ZQ et al. Lycium barbarum extract promotes M2 polarization and reduces oligomeric amyloid-β-induced inflammatory reactions in microglial cells. Neural Regen Res 17:203-209 (2022). PubMed: 34100457
- Jiang LP et al. Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice. Br J Pharmacol 179:46-64 (2022). PubMed: 34415054
- Tang G et al. Naringin Relieves Diabetic Cardiac Autonomic Neuropathy Mediated by P2Y14 Receptor in Superior Cervical Ganglion. Front Pharmacol 13:873090 (2022). PubMed: 35529431
- Wi?ckowska-Gacek A et al. Western Diet Induces Impairment of Liver-Brain Axis Accelerating Neuroinflammation and Amyloid Pathology in Alzheimer's Disease. Front Aging Neurosci 13:654509 (2021). PubMed: 33867971
- Wang L et al. P2Y12 inhibition in macrophages reduces ventricular arrhythmias in rats after myocardial ischemia-reperfusion. Adv Clin Exp Med 30:413-420 (2021). PubMed: 33908199