Overview
- Product nameAnti-P2Y6 antibody [EPR3816]See all P2Y6 primary antibodies ...
- DescriptionRabbit monoclonal [EPR3816] to P2Y6
- Tested applicationsWB, IP, IHC-P, ICC more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide corresponding to residues in Human P2Y6 (UniProt ID: Q15077).
- Positive controlA549, HACAT, HepG2, and Human spleen lysates; Human kidney tissue, Human liver tissue.
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR3816
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab92504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| IP | |
| IHC-P | |
| ICC |
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
IP: 1/50.
WB: 1/20000 - 1/100000. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionReceptor for extracellular UDP > UTP > ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
- Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
- Cellular localizationCell membrane.
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Database links
- Entrez Gene: 5031 Human
- Omim: 602451 Human
- SwissProt: Q15077 Human
- Unigene: 16362 Human
Target information above from: UniProt accession
Q15077
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- G coupled nucleotide receptor antibodyG protein coupled 6 antibodyMGC15335 antibody
- P2 purinoceptor antibodyP2RY6 antibodyP2RY6_HUMAN antibodyP2Y purinoceptor 6 antibodyP2Y6 antibodyP2Y6 receptor antibodyPP2891 antibodyPurinergic receptor P2Y6 antibodypyrimidinergic receptor P2Y antibodyPyrimidinergic receptor P2Y, G protein coupled, 6 antibody
see all
Anti-P2Y6 antibody [EPR3816] images
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![Western blot - P2Y6 antibody [EPR3816] (ab92504)](http://a.abcam.com/ps/datasheet/images/92/ab92504/P2Y6-Primary-antibodies-ab92504-1.jpg)
Western blot - P2Y6 antibody [EPR3816] (ab92504)All lanes : Anti-P2Y6 antibody [EPR3816] (ab92504) at 1/20000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HACAT cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size : 36 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P2Y6 antibody [EPR3816] (ab92504)](http://a.abcam.com/ps/datasheet/images/92/ab92504/P2Y6-Primary-antibodies-ab92504-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P2Y6 antibody [EPR3816] (ab92504)Immunohistochemical analysis of paraffin-embedded Human kidney tissue (left-hand image) and Human liver tissue (right-hand image) using ab92504 at a dilution of 1/100.
References for Anti-P2Y6 antibody [EPR3816] (ab92504)
ab92504 has not yet been referenced specifically in any publications.
