Anti-LPA-4 antibody (ab183076)
Key features and details
- Rabbit polyclonal to LPA-4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LPA-4 antibody
See all LPA-4 primary antibodies -
Description
Rabbit polyclonal to LPA-4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human LPA-4 aa 350 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Genbank: NP_005287
Database link: Q99677 -
Positive control
- Human leiomyosarcoma and liver cancer tissue lysates. Human esophagus cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/450 - 1/2000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/450 - 1/2000. Predicted molecular weight: 42 kDa. |
IHC-P
1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA. -
Tissue specificity
High expression in ovary. Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus and hippocampus. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2846 Human
- Omim: 300086 Human
- SwissProt: Q99677 Human
- Unigene: 522701 Human
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Alternative names
- 5730485F04Rik antibody
- G protein coupled receptor 23 antibody
- G-protein coupled receptor 23 antibody
see all
Images
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All lanes : Anti-LPA-4 antibody (ab183076) at 1/450 dilution
Lane 1 : Human leiomyosarcoma tissue lysate
Lane 2 : Human liver cancer tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 42 kDa
Exposure time: 30 seconds10%SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling LPA-4 with ab183076 at a 1/30 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab183076 has been referenced in 1 publication.
- Simic P et al. Glycerol-3-phosphate is an FGF23 regulator derived from the injured kidney. J Clin Invest 130:1513-1526 (2020). PubMed: 32065590