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ab114624
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab59497 for help.
There are no answered questions for ab59497
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - P4HA1 antibody (ab59497)
Anti-P4HA1 antibody (ab59497) at 0.2 µg/ml + HEK293 cell lysate (35µg protein in RIPA buffer)
Predicted band size : 61 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (ab59497)
IHC image of ab59497 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59497, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-P4HA1 antibody (ab59497)
All lanes : Anti-P4HA1 antibody (ab59497) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC12 cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 61 kDa
Observed band size : 62 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
Image courtesy of an anonymous Abreview.
P4HA1 antibody for Western blot in Mouse (59497)
Anti-P4HA1 antibody for Western blot in Rat (59497)
Anti-P4HA1 antibody for Western blot in Human (59497)
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