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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Overview
Product name
Anti-P4HA1 antibody
See all P4HA1 products (2) ...
Description
Goat polyclonal to P4HA1
Specificity
This antibody is expected to recognise both reported isoforms.
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken, Cow, Dog, Pig, Zebrafish, Orangutan
Immunogen
Synthetic peptide: C-DFARKDEPDAFKE, corresponding to internal sequence amino acids 431-443 of Human P4HA1
C-DFARKDEP DAFKE
Positive control
HEK293 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 10 µg/ml
WB: Use a concentration of 0.1 - 1 µg/ml.Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).(An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide.)
Target
Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Sequence similarities
Belongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat.
Cellular localization
Endoplasmic reticulum lumen.
Target information above from: UniProt accessionP13674
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2-oxoglutarate-4-dioxygenase subunit alpha-1 antibody
- 4-hydroxylase, alpha I subunit antibody
- 4-PH alpha-1 antibody
see all
Database links
- Entrez Gene: 5033 Human
- Entrez Gene: 18451 Mouse
- Entrez Gene: 64475 Rat
- SwissProt: P13674 Human
- SwissProt: Q60715 Mouse
- SwissProt: P54001 Rat
- Unigene: 500047 Human
- Unigene: 593005 Human
see all
Anti-P4HA1 antibody images:
Western blot - P4HA1 antibody (ab59497)
Anti-P4HA1 antibody (ab59497) at 0.2 µg/ml + HEK293 cell lysate (35µg protein in RIPA buffer)
Predicted band size : 61 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (ab59497)
IHC image of ab59497 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59497, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-P4HA1 antibody (ab59497)
ab59497 has not yet been referenced specifically in any publications.
Publishing research using ab59497? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"