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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Overview
Product name
Anti-P4HA1 antibody
See all P4HA1 products (2) ...
Description
Mouse polyclonal to P4HA1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length Human P4HA1 (NP_001017962.1)
Positive control
A-431 cell lysate and P4HA1 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 61 kDa.
Target
Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Sequence similarities
Belongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat.
Cellular localization
Endoplasmic reticulum lumen.
Target information above from: UniProt accessionP13674
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2-oxoglutarate-4-dioxygenase subunit alpha-1 antibody
- 4-hydroxylase, alpha I subunit antibody
- 4-PH alpha-1 antibody
see all
Database links
- Entrez Gene: 5033 Human
- SwissProt: P13674 Human
- Unigene: 500047 Human
- Unigene: 593005 Human
Anti-P4HA1 antibody images:
Western blot - Anti-P4HA1 antibody (ab88881)
Anti-P4HA1 antibody (ab88881) at 1 µg/ml + A-431 cell lysate at 50 µg
Predicted band size : 61 kDa
Observed band size : 61 kDa
Western blot - Anti-P4HA1 antibody (ab88881)
All lanes : Anti-P4HA1 antibody (ab88881) at 1 µg/ml
Lane 1 : P4HA1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 61 kDa
Observed band size : 61 kDa
References for Anti-P4HA1 antibody (ab88881)
This product has been referenced in:
- Dai Let al. Differential profiling studies of N-linked glycoproteins in glioblastoma cancer stem cells upon treatment with ?-secretase inhibitor. Proteomics 11:4021-8 (2011).Read more (PubMed: 21898824) »
See 1 publication for this product
Publishing research using ab88881? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"