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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab70887? Please let us know so that we can cite the reference in this datasheet
ab70887 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - P4HA2 antibody (ab70887)
All lanes : Anti-P4HA2 antibody (ab70887) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 61 kDa
Observed band size : 61 kDa
Additional bands at : 28 kDa,33 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Immunoprecipitation - P4HA2 antibody (ab70887)
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70887 at 1 µg/ml for WB subsequent to IP at 3 µg/mg lysate.
Lane 2: control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-P4HA2 antibody (ab70887)
All lanes : Anti-P4HA2 antibody (ab70887) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC12 cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 61 kDa
Observed band size : 61 kDa
Additional bands at : 35 kDa (possible degradation product).
Exposure time : 5 minutes
Good immunoreactivity observed in RIE-1 cell lysate that is consistent with the mobility of full-length P4HA2. A faster migrating species of ~35 kDa has been commonly observed with other antibodies (including this one) that could be a degradation product or alternatively spliced form of P4HA2. Further charcaterisation is required.
Image courtesy of an anonymous Abreview.
Anti-P4HA2 antibody for Western blot in Rat (70887)
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