Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-P4HA2 antibody
See all P4HA2 products (3) ...
Description
Rabbit polyclonal to P4HA2
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residue 250 and 300 of human P4HA2. (NP_004190.1)
Positive control
HeLa and 293T whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Sequence similarities
Belongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat.
Cellular localization
Endoplasmic reticulum lumen.
Target information above from: UniProt accessionO15460
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody
- 4 PH alpha 2 antibody
- 4-PH alpha-2 antibody
see all
Database links
- Entrez Gene: 8974 Human
- Omim: 600608 Human
- SwissProt: O15460 Human
- Unigene: 519568 Human
Anti-P4HA2 antibody images:
Western blot - P4HA2 antibody (ab70887)
All lanes : Anti-P4HA2 antibody (ab70887) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 61 kDa
Observed band size : 61 kDa
Additional bands at : 28 kDa,33 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Immunoprecipitation - P4HA2 antibody (ab70887)
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70887 at 1 µg/ml for WB subsequent to IP at 3 µg/mg lysate.
Lane 2: control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
References for Anti-P4HA2 antibody (ab70887)
ab70887 has not yet been referenced specifically in any publications.
Publishing research using ab70887? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"