Anti-P4HA3 antibody (ab101657)
Key features and details
- Rabbit polyclonal to P4HA3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-P4HA3 antibody -
Description
Rabbit polyclonal to P4HA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment, corresponding to internal sequence amino acids 130-396 of Human P4HA3 (BC117333).
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Positive control
- Human fetal kidney & liver lysates, Human fetal liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 61 kDa.
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IHC-P |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 61 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. -
Tissue specificity
Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions. -
Sequence similarities
Belongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat. -
Post-translational
modificationsN-glycosylation plays no role in the catalytic activity. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 283208 Human
- Entrez Gene: 320452 Mouse
- Entrez Gene: 361612 Rat
- Omim: 608987 Human
- SwissProt: Q7Z4N8 Human
- SwissProt: Q6W3F0 Mouse
- SwissProt: Q6W3E9 Rat
- Unigene: 660541 Human
see all -
Alternative names
- 2-oxoglutarate-4-dioxygenase subunit alpha-3 antibody
- 4-PH alpha-3 antibody
- P4ha3 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab101657 has been referenced in 7 publications.
- Xiao Z et al. Profiling of miR-205/P4HA3 Following Angiotensin II-Induced Atrial Fibrosis: Implications for Atrial Fibrillation. Front Cardiovasc Med 8:609300 (2021). PubMed: 33981730
- Wang J et al. Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206+ M2-like macrophage polarization. Cell Death Dis 11:136 (2020). PubMed: 32075954
- Gu X et al. Age-associated genes in human mammary gland drive human breast cancer progression. Breast Cancer Res 22:64 (2020). PubMed: 32539762
- Long R et al. COL6A6 interacted with P4HA3 to suppress the growth and metastasis of pituitary adenoma via blocking PI3K-Akt pathway. Aging (Albany NY) 11:8845-8859 (2019). PubMed: 31627190
- Song H et al. P4HA3 is Epigenetically Activated by Slug in Gastric Cancer and its Deregulation is Associated With Enhanced Metastasis and Poor Survival. Technol Cancer Res Treat 17:1533033818796485 (2018). PubMed: 30198421
- Olivares O et al. Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions. Nat Commun 8:16031 (2017). WB, IHC . PubMed: 28685754
- Akamatsu T et al. Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences. Fibrogenesis Tissue Repair 6:15 (2013). IHC-P . PubMed: 23927729