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Western blot - P4HB antibody (ab70415)
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Western blot - P4HB antibody (ab70415)
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Immunocytochemistry/ Immunofluorescence - P4HB antibody (ab70415)
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Product Name
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P4HB antibody
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See all P4HB antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to P4HB
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Immunogen
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Full length human P4HB protein (NP_000909.2)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver tissue lysate and P4HB transfected 293T lysate.
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Cellular localization
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Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Relevance
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P4HB, Prolyl 4 hydroxylase subunit beta, is a multifunctional and highly abundant enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this P4HB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for P4HB antibody images, references, products related to ab70415 and other tools.
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P4HB antibody images:
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 Western blot - P4HB antibody (ab70415)
P4HB antibody (ab70415) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 57 kDa
Observed band size : 57 kDa
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Western blot - P4HB antibody (ab70415)
All lanes : P4HB antibody (ab70415) at 1/500 dilution
Lane 1 : P4HB transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 57 kDa
Observed band size : 62 kDa (why is the actual band size different from the predicted?)
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Immunocytochemistry/ Immunofluorescence - P4HB antibody (ab70415)
ab70415 at 10µg/ml staining P4HB in Hela cells by Immunocytochemistry/Immunofluorescence.
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Search PubMed (MEDLINE) for references to P4HB
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P4HB antibody - more information
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Customer reviews (feedback) regarding P4HB antibody |
Customer FAQs regarding P4HB antibody |
Protocols for P4HB antibody |
Price and availability of products related to P4HB antibody |
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P4HB antibody - related products:
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P4HB antibody - other tools:
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Contact Abcam with a Technical Enquiry about P4HB antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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