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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P4HB antibody (ab85564)
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Product Name
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P4HB antibody
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See all P4HB antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to P4HB
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Immunogen
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Synthetic peptide conjugated to KLH, derived from the sequence around Proline 329 of Human P4HB
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Reacts with
(species key)
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Hu, Ms, Rat, Mk
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Specificity
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Recognizes endogenous levels of total P4HB.
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/50.
WB: Use at an assay dependent dilution. Predicted molecular weight: 57 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Small intestine tissue.
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Cellular localization
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Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Relevance
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P4HB, Prolyl 4 hydroxylase subunit beta, is a multifunctional and highly abundant enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 10mM sodium HEPES, 50% Glycerol, 1mg/ml BSA, 150mM Sodium chloride, pH 7.5 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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0.008 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this P4HB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for P4HB antibody images, references, products related to ab85564 and other tools.
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P4HB antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P4HB antibody (ab85564)
ab85564 at a 1/50 dilution staining P4HB in human small intestine tissue by IHC-P.
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Search PubMed (MEDLINE) for references to P4HB
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P4HB antibody - more information
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Customer reviews (feedback) regarding P4HB antibody |
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Price and availability of products related to P4HB antibody |
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P4HB antibody - related products:
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P4HB antibody - other tools:
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Contact Abcam with a Technical Enquiry about P4HB antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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