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Product Name
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P4HB protein (Active)
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Product type
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Proteins and Peptides
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Peptide
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Recombinant Full-length Human P4HB (SwissProt ID P07237) with an N-terminal 6xHis-tag is a single, non-glycosylated, polypeptide chain containing 508 amino acids and having a molecular mass of 57 kDa.
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Specificity
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Reductase Activity:
1.0 x 10-3 OD650nm/ min-2. By measuring the turbidity increase at 650 nm due to insulin reduction (Holmgren, A. (1979) J. Biol. Chem. 254, 9627–9632). The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time (Mart´nez-Galisteo, E., Padilla, C. A., Garcia-Alfonso, C., Lo´pez-Barea, J. and Barcena, J. A. (1993) Biochimie (Paris) 75, 803–809).
Isomerase Activity:
0.5 µmol active RNase A min-1 µmol P4HB-1. According to the re-activation of reduced and denatured RNase A (Lyles, M. M. and Gilbert, H. F. (1991) Biochemistry 30, 613-619).
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Tested applications
(see key)
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FuncS, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
Enzyme Assays:
Reductase Activity: Use at an assay dependent dilution.
Isomerase Activity: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
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Relevance
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P4HB, Prolyl 4 hydroxylase subunit beta, is a multifunctional and highly abundant enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
Constituents: 50mM Potassium phosphate, pH 7.5
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Purification notes
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This protein was purified by proprietary chromatographic techniques.
Purity greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
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Form
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Lyophilised
Reconstitute in sterile 18Mohm/cm water at not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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Expression system
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E. coli
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Sequence
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The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this P4HB protein (Active) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to P4HB
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