Overview

  • Product nameAnti-P70 S6 Kinase beta antibodySee all P70 S6 Kinase beta primary antibodies ...
  • Description
    Rabbit polyclonal to P70 S6 Kinase beta
  • Tested applicationsIHC-P, IP more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to amino acids 300-350 of Human P70 S6 Kinase beta (NP_003943.2)

Properties

Applications

Our Abpromise guarantee covers the use of ab70268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-6 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionPhosphorylates specifically ribosomal protein S6.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Target information above from: UniProt accession Q9UBS0 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 70 kDa ribosomal protein S6 kinase 2 antibody
      • EC 2.7.11.1 antibody
      • KS6B2_HUMAN antibody
      • p70 beta antibody
      • p70 ribosomal S6 kinase beta antibody
      • p70 S6 kinase beta antibody
      • p70 S6K-beta antibody
      • p70 S6KB antibody
      • p70 S6Kbeta antibody
      • p70(S6K) beta antibody
      • p70-beta antibody
      • p70-S6K 2 antibody
      • p70-S6KB antibody
      • P70S6K2 antibody
      • p70S6Kb antibody
      • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
      • Ribosomal protein S6 kinase beta 2 antibody
      • Ribosomal protein S6 kinase beta-2 antibody
      • Rps6kb2 antibody
      • S6 kinase related kinase antibody
      • S6 kinase-related kinase antibody
      • S6K beta 2 antibody
      • S6K beta antibody
      • S6K-beta antibody
      • S6K-beta-2 antibody
      • S6K2 antibody
      • Serine/threonine protein kinase 14 beta antibody
      • Serine/threonine-protein kinase 14 beta antibody
      • Serine/threonine-protein kinase 14B antibody
      • SRK antibody
      • STK14B antibody
      see all

    Anti-P70 S6 Kinase beta antibody images

    • IHC image of ab70268 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70268, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References for Anti-P70 S6 Kinase beta antibody (ab70268)

    ab70268 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70268.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"