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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
Overview
Product name
Anti-P70 S6 Kinase beta antibody
See all P70 S6 Kinase beta products (11) ...
Description
Rabbit polyclonal to P70 S6 Kinase beta
Tested applications
IHC-P, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide, corresponding to amino acids 300-350 of Human P70 S6 Kinase beta (NP_003943.2)
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P70 S6 Kinase beta antibody (ab70268)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab70268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP: Use at 2-6 µg/mg of lysate.
Application notes
Is unsuitable for or WB.
Target
Function
Phosphorylates specifically ribosomal protein S6.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ9UBS0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kDa ribosomal protein S6 kinase 2 antibody
- EC 2.7.11.1 antibody
- KS6B2_HUMAN antibody
see all
Database links
- Entrez Gene: 6199 Human
- Entrez Gene: 58988 Mouse
- Omim: 608939 Human
- SwissProt: P23443 Human
- SwissProt: Q9UBS0 Human
- SwissProt: Q9Z1M4 Mouse
- Unigene: 534345 Human
- Unigene: 271937 Mouse
Anti-P70 S6 Kinase beta antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P70 S6 Kinase beta antibody (ab70268)
IHC image of ab70268 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70268, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-P70 S6 Kinase beta antibody (ab70268)
ab70268 has not yet been referenced specifically in any publications.
Publishing research using ab70268? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"