Anti-P70 S6 Kinase beta antibody (ab70268)
- Product nameAnti-P70 S6 Kinase beta antibodySee all P70 S6 Kinase beta primary antibodies ...
- DescriptionRabbit polyclonal to P70 S6 Kinase beta
- Tested applicationsIHC-P, IP more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide, corresponding to amino acids 300-350 of Human P70 S6 Kinase beta (NP_003943.2)
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab70268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||IP: Use at 2-6 µg/mg of lysate.|
- FunctionPhosphorylates specifically ribosomal protein S6.
- Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
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Anti-P70 S6 Kinase beta antibody images
IHC image of ab70268 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70268, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-P70 S6 Kinase beta antibody (ab70268)
ab70268 has not yet been referenced specifically in any publications.