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Read our guarantee »Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
Anti-P70 S6 Kinase beta antibody
See all P70 S6 Kinase beta products (11) ...
Rabbit polyclonal to P70 S6 Kinase beta
IHC-P, IPmore details
Reacts with
Human
Predicted to work with
Mouse
Synthetic peptide, corresponding to amino acids 300-350 of Human P70 S6 Kinase beta (NP_003943.2)
Liquid
Store at +4°C.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
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Immunogen affinity purified
Polyclonal
IgG
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P70 S6 Kinase beta antibody (ab70268)
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Our Abpromise guarantee covers the use of ab70268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP: Use at 2-6 µg/mg of lysate.
Is unsuitable for or WB.
Phosphorylates specifically ribosomal protein S6.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ9UBS0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P70 S6 Kinase beta antibody (ab70268)

IHC image of ab70268 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70268, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab70268 has not yet been referenced specifically in any publications.
Publishing research using ab70268? Please let us know so that we can cite the reference in this datasheet
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IHC image of ab70268 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70268, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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