Overview
- Product nameAnti-P70 S6 Kinase beta antibodySee all P70 S6 Kinase beta primary antibodies ...
- DescriptionMouse monoclonal to P70 S6 Kinase beta
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Partial recombinant RPS6KB2 protein fragment with the sequence MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLE PVGHYEEVEL TETSVNVGPERIGPHSFELLRVLGKGGY GKVFQVRKVQGTNLGKIYAMKV and a proprietary tag.
- Positive controlTransfected lysate (293T cells) and recombinant protein
Properties
- FormLiquid
- Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- IsotypeIgG2a
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab76960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- FunctionPhosphorylates specifically ribosomal protein S6.
- Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. - Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Database links
- Entrez Gene: 6199 Human
- Entrez Gene: 6199 Human
- Entrez Gene: 6199 Human
- Omim: 608939 Human
- SwissProt: Q9UBS0 Human
- SwissProt: Q9UBS0 Human
- SwissProt: Q9UBS0 Human
- Unigene: 534345 Human
Target information above from: UniProt accession
Q9UBS0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 70 kDa ribosomal protein S6 kinase 2 antibodyEC 2.7.11.1 antibodyKS6B2_HUMAN antibody
- p70 beta antibodyp70 ribosomal S6 kinase beta antibodyp70 S6 kinase beta antibodyp70 S6K-beta antibodyp70 S6KB antibodyp70 S6Kbeta antibodyp70(S6K) beta antibodyp70-beta antibodyp70-S6K 2 antibodyP70S6K2 antibodyp70S6Kb antibodyRibosomal protein S6 kinase 70kDa, polypeptide 2 antibodyRibosomal protein S6 kinase beta 2 antibodyRibosomal protein S6 kinase beta-2 antibodyRps6kb2 antibodyS6 kinase related kinase antibodyS6 kinase-related kinase antibodyS6K beta 2 antibodyS6K beta antibodyS6K-beta antibodyS6K-beta-2 antibodyS6K2 antibodySerine/threonine protein kinase 14 beta antibodySerine/threonine-protein kinase 14B antibodySRK antibodySTK14B antibody
see all
Anti-P70 S6 Kinase beta antibody images
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Western blot - P70 S6 Kinase beta antibody (ab76960)Anti-P70 S6 Kinase beta antibody (ab76960) at 1/2500 dilution + Recombinant protein at 0.1 µg
Predicted band size : 53 kDa -
Western blot - P70 S6 Kinase beta antibody (ab76960)All lanes : Anti-P70 S6 Kinase beta antibody (ab76960) at 1/1000 dilution
Lane 1 : RPS6KB2 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 per lane.
Predicted band size : 53 kDa -
ELISA - P70 S6 Kinase beta antibody (ab76960)Detection limit for recombinant tagged RPS6KB2 (proprietary tag) is approximately 0.1ng/ml as a capture antibody.
References for Anti-P70 S6 Kinase beta antibody (ab76960)
ab76960 has not yet been referenced specifically in any publications.
