Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab59208? Please let us know so that we can cite the reference in this datasheet
ab59208 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunizing phosphopeptide
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of paraffin embedded human brain tissue using ab59208 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution + Lysates prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This image is a courtesy of Anonymous Abreview
Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This image is a courtesy of Anonymous Abreview
2
Call 01223 696 000 or contact us