Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
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Wrong band size in Western blot with human cancer cell lines. |
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ANSWER: |
Thanks for your phone call today and for letting us know about the trouble with ab59208 in Western blot.
As we discussed, I am sending a vial of ab48394 free of charge on the order ***. This product is in stock and should arrive tomorrow.
I hope this replacement vial will work well for you. Please keep me updated, and let me know if there is anything else that we can do for you. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunizing phosphopeptide
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of paraffin embedded human brain tissue using ab59208 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution + Lysates prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This image is a courtesy of Anonymous Abreview
Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This image is a courtesy of Anonymous Abreview
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