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Read our guarantee »Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
Anti-P70 S6 Kinase beta (phospho T229) antibody
See all P70 S6 Kinase beta products (11) ...
Rabbit polyclonal to P70 S6 Kinase beta (phospho T229)
Detects endogenous levels of P70 S6 Kinase beta only when phosphorylated at threonine 229 for human and rat, threonine 252 for mouse.
WB, IHC-P, ELISAmore details
Reacts with
Mouse, Rat, Human
Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 229 (THTPFC)
Human brain. Jurkat cells.
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.
Polyclonal
IgG
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
Our Abpromise guarantee covers the use of ab59208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P: 1/50 - 1/100.
ELISA: 1/5000
Phosphorylates specifically ribosomal protein S6.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ9UBS0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - P70 S6 Kinase beta (phospho T229) antibody (ab59208)

All lanes : Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunizing phosphopeptide
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - P70 S6 Kinase beta (phospho T229) antibody (ab59208)

Immunohistochemical analysis of paraffin embedded human brain tissue using ab59208 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
Western blot - P70 S6 Kinase beta (phospho T229) antibody (ab59208)

Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution + Lysates prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This image is a courtesy of Anonymous Abreview
Western blot - P70 S6 Kinase beta (phospho T229) antibody (ab59208)

Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This image is a courtesy of Anonymous Abreview
ab59208 has not yet been referenced specifically in any publications.
Publishing research using ab59208? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunizing phosphopeptide
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)

Immunohistochemical analysis of paraffin embedded human brain tissue using ab59208 at 1/50 dilution. Samples were treated -/+ phosphopeptide.

Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution + Lysates prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This image is a courtesy of Anonymous Abreview

Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This image is a courtesy of Anonymous Abreview
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