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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
Overview
Product name
Anti-P70 S6 Kinase beta (phospho T229) antibody
See all P70 S6 Kinase beta products (11) ...
Description
Rabbit polyclonal to P70 S6 Kinase beta (phospho T229)
Specificity
Detects endogenous levels of P70 S6 Kinase beta only when phosphorylated at threonine 229 for human and rat, threonine 252 for mouse.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 229 (THTPFC)
Positive control
Human brain. Jurkat cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
Phosphorylates specifically ribosomal protein S6.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ9UBS0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kDa ribosomal protein S6 kinase 2 antibody
- EC 2.7.11.1 antibody
- KS6B2_HUMAN antibody
see all
Database links
- Entrez Gene: 6199 Human
- Entrez Gene: 58988 Mouse
- Entrez Gene: 361696 Rat
- Omim: 608939 Human
- SwissProt: P23443 Human
- SwissProt: Q9UBS0 Human
- SwissProt: Q9Z1M4 Mouse
- Unigene: 534345 Human
see all
Anti-P70 S6 Kinase beta (phospho T229) antibody images:
Western blot - P70 S6 Kinase beta (phospho T229) antibody (ab59208)
All lanes : Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunizing phosphopeptide
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - P70 S6 Kinase beta (phospho T229) antibody (ab59208)
Immunohistochemical analysis of paraffin embedded human brain tissue using ab59208 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
Western blot - P70 S6 Kinase beta (phospho T229) antibody (ab59208)
Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution + Lysates prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This image is a courtesy of Anonymous Abreview
Western blot - P70 S6 Kinase beta (phospho T229) antibody (ab59208)
Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This image is a courtesy of Anonymous Abreview
References for Anti-P70 S6 Kinase beta (phospho T229) antibody (ab59208)
ab59208 has not yet been referenced specifically in any publications.
Publishing research using ab59208? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"