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Anti-P70 S6 Kinase beta (phospho T389) antibody (ab60948)

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Overview

Product name

Anti-P70 S6 Kinase beta (phospho T389) antibody
See all P70 S6 Kinase beta products (11) ...

Description

Rabbit polyclonal to P70 S6 Kinase beta (phospho T389)

Specificity

ab60948 detects endogenous levels of p70 S6 Kinase beta only when phosphorylated at threonine 389.

Tested applications

ICC/IF, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic phosphopeptide derived from isoform 2 of human p70 S6 Kinase beta-1 (P23443) around the phosphorylation site of threonine 389 (G-F-TP-Y-V).

Positive control

Human breast carcinoma tissue.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab60948 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P70 S6 Kinase beta (phospho T389) antibody (ab60948)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P70 S6 Kinase beta (phospho T389) antibody (ab60948) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence-Anti-P70 S6 Kinase beta (phospho T389) antibody(ab60948)Immunocytochemistry/ Immunofluorescence-Anti-P70 S6 Kinase beta (phospho T389) antibody(ab60948) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab60948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 1 µg/ml

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Function

Phosphorylates specifically ribosomal protein S6.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.

Target information above from: UniProt accessionQ9UBS0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 70 kDa ribosomal protein S6 kinase 2 antibody
  • EC 2.7.11.1 antibody
  • KS6B2_HUMAN antibody
  • p70 beta antibody
  • p70 ribosomal S6 kinase beta antibody
  • p70 S6 kinase beta antibody
  • p70 S6K-beta antibody
  • p70 S6KB antibody
  • p70 S6Kbeta antibody
  • p70(S6K) beta antibody
  • p70-beta antibody
  • p70-S6K 2 antibody
  • p70-S6KB antibody
  • P70S6K2 antibody
  • p70S6Kb antibody
  • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
  • Ribosomal protein S6 kinase beta 2 antibody
  • Ribosomal protein S6 kinase beta-2 antibody
  • Rps6kb2 antibody
  • S6 kinase related kinase antibody
  • S6 kinase-related kinase antibody
  • S6K beta 2 antibody
  • S6K beta antibody
  • S6K-beta antibody
  • S6K-beta-2 antibody
  • S6K2 antibody
  • Serine/threonine protein kinase 14 beta antibody
  • Serine/threonine-protein kinase 14 beta antibody
  • Serine/threonine-protein kinase 14B antibody
  • SRK antibody
  • STK14B antibody
see all

Database links

see all

Anti-P70 S6 Kinase beta (phospho T389) antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P70 S6 Kinase beta (phospho T389) antibody (ab60948)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - P70 S6 Kinase beta (phospho T389) antibody (ab60948)

ab60948 at 1/50 - 1/100 dilution staining P70 S6 Kinase beta in human breast carcinoma by Immunohistochemistry,
Paraffin embedded tissue, in the absence or presence of the immunising peptide.

  Immunocytochemistry/ Immunofluorescence-Anti-P70 S6 Kinase beta (phospho T389) antibody(ab60948)

Immunocytochemistry/ Immunofluorescence-Anti-P70 S6 Kinase beta (phospho T389) antibody(ab60948)

ICC/IF image of ab60948 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60948, 1µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-P70 S6 Kinase beta (phospho T389) antibody (ab60948)

ab60948 has not yet been referenced specifically in any publications.

Publishing research using ab60948? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"