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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
Overview
Product name
Anti-P70 S6 Kinase beta (phospho T421 + S424) antibody
See all P70 S6 Kinase beta products (11) ...
Description
Rabbit polyclonal to P70 S6 Kinase beta (phospho T421 + S424)
Specificity
Detects endogenous levels of Human p70 S6 Kinase beta only when phosphorylated at threonine 421 or serine 424. (The equivalent residues in other species are: Mouse: Thr444,Ser447; Rat: Thr421,Ser424)
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic phosphopeptide derived from human p70 S6 Kinase beta around the phosphorylation site of threonine 421 and serine 424(R-TP-P-V-SP).
Positive control
Extracts from NIH/3T3 cells, treated with EGF (200ng/ml, 30mins).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
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Western blot - P70 S6 Kinase beta (phospho T421 + S424) antibody (ab53029)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 80 kDa.
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Phosphorylates specifically ribosomal protein S6.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Target information above from: UniProt accessionQ9UBS0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kDa ribosomal protein S6 kinase 2 antibody
- EC 2.7.11.1 antibody
- KS6B2_HUMAN antibody
see all
Database links
- Entrez Gene: 6199 Human
- Entrez Gene: 58988 Mouse
- Entrez Gene: 361696 Rat
- Omim: 608939 Human
- SwissProt: P23443 Human
- SwissProt: Q9UBS0 Human
- SwissProt: Q9Z1M4 Mouse
- Unigene: 534345 Human
see all
Anti-P70 S6 Kinase beta (phospho T421 + S424) antibody images:
Western blot - P70 S6 Kinase beta (phospho T421 + S424) antibody (ab53029)
All lanes : Anti-P70 S6 Kinase beta (phospho T421 + S424) antibody (ab53029) at 1/500 dilution
Lane 1 : NIH 3T3 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Lane 2 : NIH 3T3 cell extract treated with EGF (200ng/ml, 30mins)
Observed band size : 79 kDa (why is the actual band size different from the predicted?)
References for Anti-P70 S6 Kinase beta (phospho T421 + S424) antibody (ab53029)
ab53029 has not yet been referenced specifically in any publications.
Publishing research using ab53029? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"