Overview

  • Product nameAnti-PAAF1 antibodySee all PAAF1 primary antibodies ...
  • Description
    Rabbit polyclonal to PAAF1
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 102-389 of Human PAAF1 (BC021541).

  • Positive control
    • HeLa and Jurkat cell lysates; Human fetal testis tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Polyclonal
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab118625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/100 - 1/500.

Target

  • FunctionInhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency.
  • Tissue specificityUbiquitously expressed, with highest levels in kidney, brain and testis.
  • Sequence similaritiesBelongs to the WD repeat PAAF1/RPN14 family.
    Contains 5 WD repeats.
  • Target information above from: UniProt accession Q9BRP4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • PAAF antibody
    • PAAF1 antibody
    • PAAF1_HUMAN antibody
    • Proteasomal ATPase-associated factor 1 antibody
    • Protein G-16 antibody
    • Rpn14 antibody
    • WD repeat domain 71 antibody
    • WD repeat-containing protein 71 antibody
    • WDR71 antibody
    see all

Anti-PAAF1 antibody images

  • All lanes : Anti-PAAF1 antibody (ab118625) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab118625, at a 1/100 dilution, staining PAAF1 in the cytoplasm of formalin fixed, paraffin embedded Human fetal testis tissue by Immunohistochemistry.

References for Anti-PAAF1 antibody (ab118625)

ab118625 has not yet been referenced specifically in any publications.

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