Anti-PABP antibody [10E10] (ab6125)

What is the isotype of this antibody? It's listed as IgG2b but other sources indicate this clone is an IgG1.

ANSWER:

Thank you again for your calls and for your patience while we have looked into your enquiry. I apologize again for the delay in getting this information for you.

My contact at the lab confirms that this antibody has an IgG2b isotype. I've looked online and there are other antibodies with the 10E10 clone number (though one is to a different protein target, Raptor). I'm not sure how to explain the discrepancy with the PABP antibody listed as an IgG1 isotype from other vendors, though most companies seem tolist this as an IgG2b clone.

I am sorry that we don'thavemore of an explain regarding this discrepancy, but if you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

What is the isotype of this antibody? It's listed as IgG2a but other sources indicate this clone is an IgG1.

ANSWER:

Thank you for your calls last week and earlier this week, and I apologize for the delay getting this information for you.

I've called over to the lab, andmy colleaguewho has more information about ab6125 has been out of the office. We should have some answers early next week.

I apologize again that this is taking so long, and we appreciate your patience. Please let me know if you have any questions or need anything else in the meantime.

I have purchased the anti-PABP antibody (ab6125) and used it successfully in western blot experiments. Recently I have attempted to use it in antibody inhibition experiments with RNA and have found that the RNA is being degraded during the experiment. I was under the impression that these antibodies were highly purified and therefore should be free of ribonucleases. It would be greatly appreciated if you have knowledge about the purity of the antibodies generated (if it is lot dependent) and if abcam or any other company guarantees their antibodies to be nuclease free.

ANSWER:

Thank you for your enquiry.

I have received information from our laboratory. The purification steps are not done aseptically (it's impossible) and so whilst I guess there could be some bacterially derived endonuclease, I think it unlikely as all our columns are stored in ethanol and all the tubing etc washed extensively before each run. Moreover, even if there were any endonucleases present, I would be surprised if they bound non specifically to protein G and so I wouldn't expect to see them in the end product. This is substantiated by the fact that we don't see any low (12-14Kd) MW bands in the preps when we run SDS-PAGE gels. Whilst we filter sterilize the preps prior to despatch, I guess if the antibody has subsequently been handled non-aseptically at any point, this could introduce bacteria which could produce endonuclease, however I think it unlikely.

I hope this information will be useful to you. Should you require any further information, please do not hesitate to contact me.

Customer received 40 ul instead of 50 ul in his vial of ab6125. Lot# 135499. Order# 101326.

ANSWER:

Thank you for your phone call and I apologize for the shortage that you received. I have arranged for a free of charge replacement vial of ab6125 to be sent to your attention. It is on order# 103119 and you should receive it Tuesday.

Please let me know if you have any additional questions or concerns.

Customer reported receiving 7 ul instead of 50 ul in her vial of ab6125. Lot# 107670. Order# 85080.

ANSWER:

Thank you for your phone call. I apologize for the shortage that you received in your vial of ab6125 and I'm sending you a replacement vial free of charge. It is on order# 86753 and you should receive this by Wednesday.

Please let me know if I can be of additional assistance.