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Western blot - PABP antibody [10E10] (ab6125)
![Western blot - PABP antibody [10E10] (ab6125)](/ps/datasheet/images/ab6125_1.jpg)
(enlarge)
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Product Name
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PABP antibody [10E10]
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See all PABP antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [10E10] to PABP
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Immunogen
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Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI.
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Reacts with
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Hu, Chk, Rb, Xl
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Does not react with
(species key)
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Ms, Dm
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Tested applications
(see key)
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ELISA, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution (PMID 17977970).
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic. Shuttles between the cytoplasm and the nucleus.
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> P bodies/GW bodies
Chromatin and Nuclear Signaling >> RNAi >> Dicer
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Relevance
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The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. Grange et al. (1987) isolated a melanoma cell cDNA encoding human PABP. The predicted 633-amino acid protein contains 4 repeats of an approximately 80-amino acid unit in its N-terminal half. The authors found that this repeat region is highly conserved between human and yeast PABP and is sufficient for poly(A) binding. In vitro translation of the human PABP cDNA yielded a protein with an apparent molecular mass of 73 kD by SDS-PAGE. Northern blot analysis indicated that PABP is expressed as a 2.9-kb mRNA in human melanoma cells. Gorlach et al. (1994) noted that each of the 4 repeats of PABP is a ribonucleoprotein (RNP) consensus sequence RNA-binding domain. They determined that PABP has a pI of approximately 10.3 and is a very abundant, stable protein. Immunofluorescence studies of mammalian cells indicated that PABP is located exclusively in the cytoplasm. However, using both indirect immunofluorescence and tagging of PABP1 by fusion to the green fluorescent protein (GFP), Afonina et al. (1998) demonstrated that PABP1 shuttles between the nucleus and cytoplasm. PABP1 accumulated in the nucleus when transcription was inhibited, suggesting that active transcription is required for nuclear export of PABP1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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10E10
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Myeloma
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Sp2/0
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Isotype
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IgG2b
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from supernatant.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PABP antibody [10E10] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PABP antibody [10E10] images, references, products related to ab6125 and other tools.
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PABP antibody [10E10] images:
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 Western blot - PABP antibody [10E10] (ab6125)
All lanes : PABP antibody [10E10] (ab6125) at 1/2000 dilution
Lane 1 : 10ug of protein from MCF10A cells transfected with negative control siRNA.
Lane 2 : 10ug of protein from MCF10A cells transfected with PABP specific siRNA.
Lane 3 : 10 ug of protein from MCF10A cells transfected with PABP specific siRNA.
Secondary
Goat anti-mouse 1/10000
Predicted band size : 71 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
The cells were lysed with NP40 buffer with protease inhibitor cocktail, 10 micrograms of protein were separated by SDS-PAGE and transferred to PVDF membrane, blocked and blotted for two hours with PABP antibody in TBST, washed three times, secondary antibody for 1 hr (goat anti-mouse, Amersham, 1:10000). From an Abreview by Francisco Ramirez-Valle
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References for PABP antibody [10E10] (ab6125)
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This product has been used in: (two most recent references)
Castell A et al. HIV- 1 protease inhibits Cap- and poly(A)-dependent translation upon eIF4GI and PABP cleavage. PLoS One 4:e7997 (2009). WB; Human. PubMed: 19956697
Marin M et al. Human immunodeficiency virus type 1 Vif functionally interacts with diverse APOBEC3 cytidine deaminases and moves with them between cytoplasmic sites of mRNA metabolism. J Virol 82:987-98 (2008). ICC/IF; Human. PubMed: 17977970
See all 9 publication references for this product.
If you publish research using ab6125 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PABP
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PABP antibody [10E10] - more information
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Customer reviews (feedback) regarding PABP antibody [10E10] |
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PABP antibody [10E10] - related products:
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PABP antibody [10E10] - other tools:
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Contact Abcam with a Technical Enquiry about PABP antibody [10E10] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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