Anti-PABP antibody (ab21060)
Key features and details
- Rabbit polyclonal to PABP
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PABP antibody
See all PABP primary antibodies -
Description
Rabbit polyclonal to PABP -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab312314.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human PABP.
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Positive control
- ICC/IF: HeLa cells. WB: HeLa, NIH/3T3, MEF1 and PC12 whole cell lysate. Mouse brain, testis, spinal cord, ovary and pancreas tissue lysate. Brain (Rat) Tissue Lysate - normal tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa.
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ICC/IF | (4) |
Use a concentration of 1 - 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299). Positively regulates the replication of dengue virus (DENV) (PubMed:26735137). -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the polyadenylate-binding protein type-1 family.
Contains 1 PABC domain.
Contains 4 RRM (RNA recognition motif) domains. -
Domain
The RNA-binding domains RRM1 and RRM2 and the C-terminus (last 138 amino acids) regions interact with the PABPC1-interacting motif-1 (PAM1) and -2 (PAM2) of PAIP1, respectively.
The RNA-binding domains RRM2 and RRM3 and the C-terminus (last 138 amino acids) regions interact with the PABPC1-interacting motif-1 (PAM1) and -2 (PAM2) of PAIP2, respectively. -
Post-translational
modificationsPhosphorylated by MAPKAPK2.
Methylated by CARM1. Arg-493 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. Nucleus. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the cytoplasm and the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 26986 Human
- Entrez Gene: 18458 Mouse
- Entrez Gene: 171350 Rat
- Omim: 604679 Human
- SwissProt: P11940 Human
- SwissProt: P29341 Mouse
- SwissProt: Q9EPH8 Rat
- Unigene: 387804 Human
see all -
Alternative names
- PAB 1 antibody
- PAB1 antibody
- PABP 1 antibody
see all
Images
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ab21060 staining PABP in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab21060 at 1 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Anti-PABP antibody (ab21060) at 1 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg/ml
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?ab21060 detects a band of ~ 75 kDa in HeLa whole cell lysate. A 75 kDa band is also seen in Jurkat and A431 whole cell lysates (data not shown). PABP is reported to have run at an apparent molecular mass of 73 kDa on SDS-PAGE. We believe the band we see at 75 kDa represents PABP.
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Viral ORF57 expression is required to suppress the formation of SG during KSHV lytic infection and under arsenite stress.
ORF57 alone is sufficient to inhibit SG formation. HeLa cells transfected with an ORF57-Flag (pVM7) expressing vector or an empty vector (control) for 24 h were induced with 0.5 mM arsenite for 30 min for SG formation. The cells were stained for ORF57 (green), SG-specific TIA-1 (red) and PABPC1 (white) (ab21060) by each corresponding antibody. The nuclei were counterstained with Hoechst stain. Scale bar = 10 μm.
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All lanes : Anti-PABP antibody (ab21060) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 :Mouse pancreas tissue lysate - total protein (ab29363)
Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 6 : Spinal Cord (Mouse) Tissue Lysate
Lane 7 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 8 : PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 9 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands. -
ab21060 at 1/50 staining HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF.
HeLa cells fixed for 10 minutes with 1.9 % formaldehyde in 2 % sucrose in PBS then permeabilized for 10 minutes with 0.5% NP40 in PBS-10% sucrose. The cells were then blocked in horse serum and incubated with the antibody for 1 hour. An Alexa Fluor ® 555 conjugated donkey anti-rabbit antibody was used as the secondary.
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Immunocytochemical analysis of newborn porcine tracheal epithelial cells, labeling PABP with ab21060.
Cells were formaldehyde-fixed, permeabilized in cold methanol, and blocked with 5% horse serum in PBS for 40 minutes at 23°C. Immunostaining with primary anti-PABP diluted 1/250 for 12 hours at 4°C.
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Immunocytochemical analysis of rat OLN93 oligodendroglia cells, labeling PABP with ab21060.
Cells were paraformaldehyde-fixed, permeabilized with 0.1% Triton X-100, and blocked with 10% normal goat serum for 1 hour at 20°C. Immunostaining with anti-PABP diluted 1/200 overnight at 4°C.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (120)
ab21060 has been referenced in 120 publications.
- Luqman-Fatah A et al. The interferon stimulated gene-encoded protein HELZ2 inhibits human LINE-1 retrotransposition and LINE-1 RNA-mediated type I interferon induction. Nat Commun 14:203 (2023). PubMed: 36639706
- Alvarado-Hernandez B et al. Protein-RNA Interactome Analysis Reveals Wide Association of Kaposi's Sarcoma-Associated Herpesvirus ORF57 with Host Noncoding RNAs and Polysomes. J Virol 96:e0178221 (2022). PubMed: 34787459
- Takahashi M et al. USP10 Inhibits Aberrant Cytoplasmic Aggregation of TDP-43 by Promoting Stress Granule Clearance. Mol Cell Biol 42:e0039321 (2022). PubMed: 35007165
- Wu AC et al. HDAC6 involves in regulating the lncRNA-microRNA-mRNA network to promote the proliferation of glioblastoma cells. J Exp Clin Cancer Res 41:47 (2022). PubMed: 35109908
- Stoneley M et al. Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress. Mol Cell 82:1557-1572.e7 (2022). PubMed: 35180429