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All lanes : Anti-PABP2 antibody [EP3001Y] (ab81224) at 1/20000 dilution
Lane 1 : T47D cell
lysates
Lane 2 : SKBR3 cell
lysates
Lane 3 : Ramos cell
lysates
Lane 4 : Raji cell
lysates
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size : 33 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry staining of paraffin-embedded urinary bladder transitional cell carcinoma tissue, using 1/100 ab81224
0
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