You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-PABP2 antibody [EP3001Y] (ab81224)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-PABP2 antibody [EP3001Y]
See all PABP2 products (2) ...

Description

Rabbit monoclonal [EP3001Y] to PABP2

Tested applications

WB, IHC-P, ICC, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Human

Does not react with

Rat

Immunogen

Synthetic peptide from Human PABP2

Positive control

WB: T47D, SKBR3, Ramos and Raji cell lysates; IHC: urinary bladder transitional cell carcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP3001Y

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> RNAi >> P bodies/GW bodies
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding

  • Western blot - PABP2 antibody [EP3001Y] (ab81224)Western blot - PABP2 antibody [EP3001Y] (ab81224) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABP2 antibody [EP3001Y] (ab81224)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABP2 antibody [EP3001Y] (ab81224) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab81224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/10000 - 1/50000.Pr...Read more →

    WB: 1/10000 - 1/50000.Predicted molecular weight: 33 kDa.

  • 1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.

    IHC-P: 1/100 - 1/250.

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

  • Flow Cyt

     Flow Cyt: 1/60

    Flow Cyt: 1/60

Application notes

Is unsuitable for or IP.

Target

Relevance

PABP2 is involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. Two different isoform have been identified.

Cellular localization

Cytoplasmic and Nuclear

Alternative names

  • Nuclear poly(A) binding protein 1 antibody
  • PAB2 antibody
  • PABII antibody
  • PABP2 antibody
  • PABPN1 antibody
  • Poly(A) binding protein 2 antibody
  • Polyadenylate binding nuclear protein 1 antibody
  • Polyadenylate binding protein 2 antibody
  • ROX2 antibody
see all

Database links

Anti-PABP2 antibody [EP3001Y] images:

  Western blot - PABP2 antibody [EP3001Y] (ab81224)

Western blot - PABP2 antibody [EP3001Y] (ab81224)

All lanes : Anti-PABP2 antibody [EP3001Y] (ab81224) at 1/20000 dilution

Lane 1 : T47D cell lysates
Lane 2 : SKBR3 cell lysates
Lane 3 : Ramos cell lysates
Lane 4 : Raji cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size : 33 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABP2 antibody [EP3001Y] (ab81224)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABP2 antibody [EP3001Y] (ab81224)

Immunohistochemistry staining of paraffin-embedded urinary bladder transitional cell carcinoma tissue, using 1/100 ab81224

References for Anti-PABP2 antibody [EP3001Y] (ab81224)

This product has been referenced in:

  • Kim SHet al. ALS-associated proteins TDP-43 and FUS/TLS function in a common biochemical complex to coregulate HDAC6 mRNA. J Biol Chem : (2010). WB; Human.Read more (PubMed: 20720006) »
  • Kim SHet al. Amyotrophic lateral sclerosis-associated proteins TDP-43 and FUS/TLS function in a common biochemical complex to co-regulate HDAC6 mRNA. J Biol Chem 285:34097-105 (2010).Read more (PubMed: 20720006) »

See all 2 publications for this product

Publishing research using ab81224? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"