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Western blot - PABP2 antibody [EP3001Y] (ab81224)
![Western blot - PABP2 antibody [EP3001Y] (ab81224)](/ps/datasheet/images/81/ab81224/PABP2-Primary-antibodies-ab81224-3.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABP2 antibody [EP3001Y] (ab81224)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABP2 antibody [EP3001Y] (ab81224)](/ps/datasheet/images/81/ab81224/PABP2-Primary-antibodies-ab81224-4.jpg)
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Product Name
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PABP2 antibody [EP3001Y]
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See all PABP2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP3001Y] to PABP2
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Immunogen
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Synthetic peptide from Human PABP2
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Reacts with
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Hu, Ms
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Does not react with
(species key)
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Rat
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/60.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250.
WB: 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: T47D, SKBR3, Ramos and Raji cell lysates;
IHC: urinary bladder transitional cell carcinoma.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> P bodies/GW bodies
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
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Relevance
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PABP2 is involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. Two different isoform have been identified.
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PABP2 antibody [EP3001Y] (ab81224) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP3001Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PABP2 antibody [EP3001Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PABP2 antibody [EP3001Y] images, references, products related to ab81224 and other tools.
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PABP2 antibody [EP3001Y] images:
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 Western blot - PABP2 antibody [EP3001Y] (ab81224)
All lanes : PABP2 antibody [EP3001Y] (ab81224) at 1/20000 dilution
Lane 1 : T47D cell
lysates
Lane 2 : SKBR3 cell
lysates
Lane 3 : Ramos cell
lysates
Lane 4 : Raji cell
lysates
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size : 33 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABP2 antibody [EP3001Y] (ab81224)
Immunohistochemistry staining of paraffin-embedded urinary
bladder transitional cell carcinoma tissue, using 1/100 ab81224
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Search PubMed (MEDLINE) for references to PABP2
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PABP2 antibody [EP3001Y] - more information
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Customer reviews (feedback) regarding PABP2 antibody [EP3001Y] |
Customer FAQs regarding PABP2 antibody [EP3001Y] |
Protocols for PABP2 antibody [EP3001Y] |
Price and availability of products related to PABP2 antibody [EP3001Y] |
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PABP2 antibody [EP3001Y] - related products:
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PABP2 antibody [EP3001Y] - other tools:
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Contact Abcam with a Technical Enquiry about PABP2 antibody [EP3001Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - PABP2 antibody [EP3001Y] (ab81224)](/index.html?datasheet=81224#PABP2-Primary-antibodies-ab81224-3.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABP2 antibody [EP3001Y] (ab81224)](/index.html?datasheet=81224#PABP2-Primary-antibodies-ab81224-4.jpg)
