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Western blot - PABP2 antibody (ab21866)
(enlarge)
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Product Name
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PABP2 antibody
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See all PABP2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to PABP2
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Immunogen
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Fusion protein: DEDITLNEDQ LLESLEETNG EQETEIATEV EEEGSMQIDP ELEAIKARVK EMEEEAEKIK QMQSEVDKQM AGGSTTGLAT VPLSLEEKQE IDTRSVYVGN corresponding to amino acids 3/102 of Drosophila melanogaster PABP2.
BLAST 'DEDITLNEDQ LLESLEETNG EQETEIATEV EEEGSMQIDP ELEAIKARVK EMEEEAEKIK QMQSEVDKQM AGGSTTGLAT VPLSLEEKQE IDTRSVYVGN ' with  or 
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Reacts with
(species key)
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Dm
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 25 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> P bodies/GW bodies
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
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Relevance
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PABP2 is involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. Two different isoform have been identified.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 35788 (Fruit fly (Drosophila melanogaster))
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| SwissProt | | Q7KNF2 (Fruit fly (Drosophila melanogaster))
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Constituents: 50% Glycerol Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Notes
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Produced from outbred CD1 mice
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PABP2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PABP2 antibody images, references, products related to ab21866 and other tools.
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PABP2 antibody images:
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 Western blot - PABP2 antibody (ab21866)
All lanes : PABP2 antibody (ab21866) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 25 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
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Search PubMed (MEDLINE) for references to PABP2
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PABP2 antibody - more information
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Customer reviews (feedback) regarding PABP2 antibody |
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PABP2 antibody - other tools:
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