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Overview
Product name
Anti-PABP2 antibody
See all PABP2 products (2) ...
Description
Mouse polyclonal to PABP2
Tested applications
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Fruit fly (Drosophila melanogaster)
Immunogen
Fusion protein: DEDITLNEDQLLESLEETNGEQETEIATEVEEEGSMQIDP ELEAIKARVKEMEEEAEKIKQMQSEVDKQMAGGSTTGLAT VPLSLEEKQEIDTRSVYVGN, corresponding to amino acids 3/102 of Drosophila melanogaster PABP2.
DEDITLNEDQ LLESLEETNG EQETEIATEV EEEGSMQIDP ELEAIKARVK EMEEEAEKIK QMQSEVDKQM AGGSTTGLAT VPLSLEEKQE IDTRSVYVGN
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - PABP2 antibody (ab21866)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 25 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Relevance
PABP2 is involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. Two different isoform have been identified.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- Cg2163-pa isoform a antibody
- CG2163-PB isoform B antibody
- Nuclear poly(A) binding protein 1 antibody
see all
Database links
- Entrez Gene: 35788 Fruit fly (Drosophila melanogaster)
- SwissProt: Q7KNF2 Fruit fly (Drosophila melanogaster)
Anti-PABP2 antibody images:
Western blot - PABP2 antibody (ab21866)
All lanes : Anti-PABP2 antibody (ab21866) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 25 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
References for Anti-PABP2 antibody (ab21866)
ab21866 has not yet been referenced specifically in any publications.
Publishing research using ab21866? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"