Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-PABPC4 antibody
See all PABPC4 products (3) ...
Description
Rabbit polyclonal to PABPC4
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide: AELGAKAKEF TNVYIKNFGE EVDDESLKEL FSQFGKTLSV KVMRDPNGKS, corresponding to a region within amino acids 181-230 of Human PABPC4.
AELGAKAKEF TNVYIKNFGE EVDDESLKEL FSQFGKTLSV KVMRDPNGKS
Positive control
Human kidney tissue. HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
IHC-P: Use a concentration of 4.0 - 8.0 µg/ml.
Target
Relevance
Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and a proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.
Cellular localization
Cytoplasmic
Alternative names
- Activated platelet protein 1 antibody
- APP 1 antibody
- APP1 antibody
see all
Database links
- Entrez Gene: 534576 Cow
- Entrez Gene: 482464 Dog
- Entrez Gene: 8761 Human
- Entrez Gene: 230721 Mouse
- Entrez Gene: 298510 Rat
- Omim: 603407 Human
- SwissProt: Q13310 Human
- Unigene: 169900 Human
see all
Anti-PABPC4 antibody images:
Western blot - PABPC4 antibody (ab66897)
All lanes : Anti-PABPC4 antibody (ab66897) at 1.25 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 71 kDa
Observed band size : 71 kDa
Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABPC4 antibody (ab66897)
ab66897, at 4µg/ml, staining PABPC4 in human kidney by immunohistochemistry using paraffin-embedded tissue. The arrows indicate positively stained epithelial cells of the renal tubule.
References for Anti-PABPC4 antibody (ab66897)
ab66897 has not yet been referenced specifically in any publications.
Publishing research using ab66897? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"