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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab75855 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - PABPN1 antibody [EP3000Y] (ab75855)](/ps/datasheet/Images/75/ab75855/ab75855_WB_1.jpg)
Western blot - PABPN1 antibody [EP3000Y] (ab75855)
Lanes 1 - 2 : Anti-PABPN1 antibody [EP3000Y] (ab75855) at 1/200000 dilution
Lane 3 : Anti-PABPN1 antibody [EP3000Y] (ab75855) at 1/1000000 dilution
Lane 1 : Raw264.7 cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size : 33 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABPN1 antibody [EP3000Y] (ab75855)](/ps/datasheet/Images/75/ab75855/ab75855_IHC_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABPN1 antibody [EP3000Y] (ab75855)
ab75855, at 1/100 dilution, staining PABPN1 in squamous cell cervical carcinoma, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
![Immunocytochemistry/ Immunofluorescence - PABPN1 antibody [EP3000Y] (ab75855)](/ps/datasheet/Images/75/ab75855/ab75855_IF_1.jpg)
Immunocytochemistry/ Immunofluorescence - PABPN1 antibody [EP3000Y] (ab75855)
ab75855, at 1/100 dilution, staining PABPN1 in HeLa cells, by Immunofluorescent staining.
![Flow Cytometry - Anti-PABPN1 antibody [EP3000Y] (ab75855)](/ps/datasheet/images/75/ab75855/PABPN1-Primary-antibodies-ab75855-2.jpg)
Flow Cytometry - Anti-PABPN1 antibody [EP3000Y] (ab75855)
Overlay histogram showing MCF-7 cells stained with ab75855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75855, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
![PABPN1 antibody [EP3000Y] for WB in Human (75855)](/ps/datasheet/images/75/ab75855/PABPN1-Primary-antibodies-ab75855-1.jpg)
PABPN1 antibody [EP3000Y] for WB in Human (75855)
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