Anti-PABPN1 antibody (ab130654)

Overview

• Product nameAnti-PABPN1 antibodySee all PABPN1 primary antibodies ...
• Description
  Rabbit polyclonal to PABPN1
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 269-298 of Human PABPN1 (NP_004634.1).

• Positive controlHela cell line lysate

Properties

• FormLiquid
• Storage instructionsStore at 4°C (up to 6 months). For long term storage store at -20°C
• Storage bufferPreservative: 0.09% Sodium azide
  Constituent: 99% PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab130654 is purified through a protein A column, followed by peptide affinity purification.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Other

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Splicing

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • Chromatin Binding Proteins
  • DNA / RNA binding

Applications

Target

• FunctionInvolved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.
• Tissue specificityUbiquitous.
• Involvement in diseaseDefects in PABPN1 are the cause of oculopharyngeal muscular dystrophy (OPMD) [MIM:164300]. OPMD is a form of late-onset slowly progressive myopathy characterized by eyelid ptosis, dysphagia and, sometimes by other cranial and limb-muscle involvement.
• Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
• DomainThe RRM domain is essential for specific adenine bases recognition in the poly(A) tail but not sufficient for poly(A) binding.
• Post-translational
  modificationsArginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties.
• Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus. Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP-sensitive import mechanism. Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Nucleus; nuclear speckle. Colocalizes with SKIP and poly(A) RNA in nuclear speckles.

Target information above from: UniProt accession Q86U42 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8106 Human
  • Omim: 602279 Human
  • SwissProt: Q86U42 Human
  • Unigene: 117176 Human
  • Unigene: 707712 Human

• Alternative names
  Nuclear poly(A)-binding protein 1 antibodyOPMD antibodyPAB2 antibody

  PABII antibodyPABP 2 antibodypABP-2 antibodyPABP2 antibodyPABP2_HUMAN antibodyPABPII antibodyPabpn1 antibodypoly(A) binding protein nuclear 1 antibodyPoly(A)-binding protein 2 antibodyPoly(A)-binding protein II antibodyPolyA binding protein II antibodyPolyadenylate-binding nuclear protein 1 antibodyPolyadenylate-binding protein 2 antibody

  see all

Anti-PABPN1 antibody images

• 

  Western blot - Anti-PABPN1 antibody (ab130654)
  Anti-PABPN1 antibody (ab130654) at 1/100 dilution + Hela cell line lysate at 35 µg
  
  Predicted band size : 33 kDa