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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Overview
Product name
Anti-PACE4 antibody - Aminoterminal end
See all PACE4 products (5) ...
Description
Rabbit polyclonal to PACE4 - Aminoterminal end
Specificity
ab39877 recognizes the amino end of mature enzyme PACE4 and all of its splice variants.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the amino end of mature Human PACE4.
(Peptide available as ab41417.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG1
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
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Western blot - PACE4 antibody - Aminoterminal end (ab39877)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IHC-P
Application notes
IHC-P: 1/500 (PMID 19339735).
WB: 1/1000 - 1/5000. Predicted molecular weight: 106 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
Tissue specificity
Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 homo B/P domain.
Contains 1 PLAC domain.
Domain
The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
Cellular localization
Secreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.
Target information above from: UniProt accessionP29122
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- paired basic amino acid cleaving enzyme 4 antibody
- paired basic amino acid cleaving system 4 antibody
- Pcsk6 antibody
see all
Database links
- Entrez Gene: 5046 Human
- Omim: 167405 Human
- SwissProt: P29122 Human
- Unigene: 498494 Human
- Unigene: 665989 Human
Anti-PACE4 antibody - Aminoterminal end images:
Western blot - PACE4 antibody - Aminoterminal end (ab39877)
All lanes : Anti-PACE4 antibody - Aminoterminal end (ab39877) at 1/1000 dilution
Lane 1 : SF treated PK15 CM
Lane 2 : TPA treated PK15 CM
Lane 3 : IL1-beta treated PK15 CM
Lysates/proteins at 30 µl per lane.
Predicted band size : 106 kDa
Observed band size : 56 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-PACE4 antibody - Aminoterminal end (ab39877)
This product has been referenced in:
- Fuller JAet al. Subtilisin-like proprotein convertase expression, localization, and activity in the human retina and optic nerve head. Invest Ophthalmol Vis Sci 50:5759-68 (2009). WB, IHC-P; Human.Read more (PubMed: 19339735) »
See 1 publication for this product
Publishing research using ab39877? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"