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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Overview
Product name
Anti-PACE4 antibody
See all PACE4 products (5) ...
Description
Mouse monoclonal to PACE4
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 860-970 of Human PACE4
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
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Western blot - PACE4 antibody (ab58226)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
Tissue specificity
Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 homo B/P domain.
Contains 1 PLAC domain.
Domain
The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
Cellular localization
Secreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.
Target information above from: UniProt accessionP29122
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Paired basic amino acid cleaving enzyme 4 antibody
- paired basic amino acid cleaving system 4 antibody
- PCSK6 antibody
see all
Database links
- Entrez Gene: 5046 Human
- Omim: 167405 Human
- SwissProt: P29122 Human
- Unigene: 498494 Human
- Unigene: 665989 Human
Anti-PACE4 antibody images:
Western blot - PACE4 antibody (ab58226)
Western blot against tagged recombinant protein immunogen using ab58226 PACE4 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
References for Anti-PACE4 antibody (ab58226)
ab58226 has not yet been referenced specifically in any publications.
Publishing research using ab58226? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"