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Anti-PACRG antibody (ab4090)

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Overview

Product name

Anti-PACRG antibody
See all PACRG products (2) ...

Description

Rabbit polyclonal to PACRG

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Zebrafish

Immunogen

Synthetic peptide: CNVNSGDGIDYSQ, corresponding to amino acids 188 - 199 of Human PACRG.

CNVNSGDGID YSQ

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Parkin / PARK

  • Western blot - PACRG antibody (ab4090)Western blot - PACRG antibody (ab4090) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/3000.Detec...Read more →

    WB: 1/500 - 1/3000.Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).

Target

Relevance

PACRG is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoter between the two genes.

Alternative names

  • FLJ32724 antibody
  • GLUP antibody
  • HAK005771 antibody
  • Molecular chaperone/chaperonin binding protein antibody
  • PARK 2 co regulated antibody
  • PARK 2 coregulated antibody
  • PARK2 co regulated antibody
  • PARK2 coregulated antibody
  • PARK2CRG antibody
  • Parkin co regulated gene protein antibody
  • Parkin Co-regulated Gene antibody
  • Parkin coregulated gene protein antibody
  • RP3 495O10.2 antibody
see all

Database links

Anti-PACRG antibody images:

  Western blot - PACRG antibody (ab4090)

Western blot - PACRG antibody (ab4090)

All lanes : Anti-PACRG antibody (ab4090) at 1/1500 dilution

Lane 1 : Mouse embryonic fibroblast (MEF) whole cell lysate
Lane 2 : Mouse embryonic fibroblast (MEF) whole cell lysate with immunising peptide

Lysates/proteins at 35 µg per lane.

Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size : 33 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-PACRG antibody (ab4090)

This product has been referenced in:

  • Gavino C & Richard S Patched1 haploinsufficiency impairs ependymal cilia function of the quaking viable mice, leading to fatal hydrocephalus. Mol Cell Neurosci 47:100-7 (2011).Read more (PubMed: 21447392) »

See 1 publication for this product

Publishing research using ab4090? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"