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Western blot - PACRG antibody (ab4090)
(enlarge)
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Product Name
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PACRG antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PACRG
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Immunogen
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Synthetic peptide: CNVNSGDGIDYSQ, corresponding to amino acids 188 - 199 of Human PACRG.
BLAST 'CNVNSGDGIDYSQ' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Chk, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/3000. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Parkin / PARK
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Relevance
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PACRG is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoter between the two genes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
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Form
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Liquid
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Concentration
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1.600 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PACRG antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PACRG antibody images, references, products related to ab4090 and other tools.
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PACRG antibody images:
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 Western blot - PACRG antibody (ab4090)
All lanes : PACRG antibody (ab4090) at 1/1500 dilution
Lane 1 : Mouse embryonic fibroblast (MEF) whole cell lysate
Lane 2 : Mouse embryonic fibroblast (MEF) whole cell lysate with immunising peptide
Lysates/proteins at 35 µg per lane.
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size : 33 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to PACRG
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PACRG antibody - more information
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Customer reviews (feedback) regarding PACRG antibody |
Customer FAQs regarding PACRG antibody |
Protocols for PACRG antibody |
Price and availability of products related to PACRG antibody |
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PACRG antibody - related products:
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PACRG antibody - other tools:
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Contact Abcam with a Technical Enquiry about PACRG antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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