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Read our guarantee »Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Anti-PACS1 antibody
Rabbit polyclonal to PACS1
WB, ELISAmore details
Reacts with
Human
Predicted to work with
Mouse, Rat
Synthetic peptide corresponding to C terminal residues of human PACS1 (Phosphofurin acidic cluster sorting protein 1)
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
Immunogen affinity purified
Purified by antigen specific affinity chromatography.
Polyclonal
IgG
Western blot - PACS1 antibody (ab56072)
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Our Abpromise guarantee covers the use of ab56072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml for 2hrs. Predicted molecular weight: 105 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
PACS1 is a coat protein that is involved in the localization of trans Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs. In addition, the human protein plays a role in HIV-1 Nef-mediated downregulation of cell surface MHC-I molecules to the TGN, thereby enabling HIV-1 to escape immune surveillance. PACS1 is known to interact with HIV-1 Nef, AP-1 and AP-3 but not with AP-2.
Golgi apparatus, trans Golgi network.
Western blot - PACS1 antibody (ab56072)

Predicted band size : 105 kDa
ab56072 used in Western Blot.Approximately 2 × 106 (HeLa or Jurkat) cells were disrupted in 0.5 ml of lysis buffer, and initially 50 μl of 15,000 × g supernatants were immunoblotted for actin. After adjusting the volume to constant actin values, the indicated vesicular or adapter proteins were detected by immunoblotting extracts of cells treated with the siRNAs listed below the respective blots.
Image from Yi L et al, J Biol Chem. 2010 Oct 1;285(40):30884-905. Epub 2010 Jul 9, Fig 4. DOI 10.1074/jbc.M110.135947
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Predicted band size : 105 kDa
ab56072 used in Western Blot.Approximately 2 × 106 (HeLa or Jurkat) cells were disrupted in 0.5 ml of lysis buffer, and initially 50 μl of 15,000 × g supernatants were immunoblotted for actin. After adjusting the volume to constant actin values, the indicated vesicular or adapter proteins were detected by immunoblotting extracts of cells treated with the siRNAs listed below the respective blots.
Image from Yi L et al, J Biol Chem. 2010 Oct 1;285(40):30884-905. Epub 2010 Jul 9, Fig 4. DOI 10.1074/jbc.M110.135947
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