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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-PACSIN3 antibody
See all PACSIN3 products (4) ...
Description
Goat polyclonal to PACSIN3
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: APEEDAGGEALGGS, corresponding to amino acids 2-15 of Human PACSIN3.
APEEDAGGEA LGGS-C
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.2% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Western blot - PACSIN3 antibody (ab2226)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/16000(This has only been tested against the recombinant protein.)
WB: Use a concentration of 0.3 µg/mlDetects a band of approximately 50 kDa.
Target
Function
May play a role in vesicle formation and transport.
Tissue specificity
Expressed in heart and skeletal muscle, lung, kidney, adrenal gland and placenta.
Sequence similarities
Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain.
Post-translational
modifications
Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
Cellular localization
Cytoplasm. Cell membrane. Detected at the inner aspect of the plasma membrane in cells or myotubes.
Target information above from: UniProt accessionQ9UKS6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Endophilin 1 antibody
- Endophilin I antibody
- FLJ17861 antibody
see all
Database links
- Entrez Gene: 29763 Human
- Entrez Gene: 80708 Mouse
- Entrez Gene: 311187 Rat
- Omim: 606513 Human
- SwissProt: Q9UKS6 Human
- SwissProt: Q99JB8 Mouse
- SwissProt: Q5I2Z0 Rat
- Unigene: 334639 Human
Anti-PACSIN3 antibody images:
Western blot - PACSIN3 antibody (ab2226)
Western Blot staining for ab2226 (1µg/ml) of H460 lysate (35µg total protein per lane). Primary incubated for 1 hour.
References for Anti-PACSIN3 antibody (ab2226)
This product has been referenced in:
- Roach W & Plomann M PACSIN3 overexpression increases adipocyte glucose transport through GLUT1. Biochem Biophys Res Commun 355:745-50 (2007). WB; Human.Read more (PubMed: 17320047) »
See 1 publication for this product
Publishing research using ab2226? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"